147214 20241216115435.0 doi 10.1021/nn505468v sideral 89780 ART-2015-89780 eng Pérez-Hernández, M. Dissecting the molecular mechanism of apoptosis during photothermal therapy using gold nanoprisms 2015 The photothermal response of plasmonic nanomaterials can be exploited for a number of biomedical applications in diagnostics (biosensing and optoacoustic imaging) and therapy (drug delivery and photothermal therapy). The most common cellular response to photothermal cancer treatment (ablation of solid tumors) using plasmonic nanomaterials is necrosis, a process that releases intracellular constituents into the extracellular milieu producing detrimental inflammatory responses. Here we report the use of laser-induced photothermal therapy employing gold nanoprisms (NPRs) to specifically induce apoptosis in mouse embryonic fibroblast cells transformed with the SV40 virus. Laser-irradiated "hot" NPRs activate the intrinsic/mitochondrial pathway of apoptosis (programmed cell death), which is mediated by the nuclear-encoded proteins Bak and Bax through the activation of the BH3-only protein Bid. We confirm that an apoptosis mechanism is responsible by showing how the NPR-mediated cell death is dependent on the presence of caspase-9 and caspase-3 proteins. The ability to selectively induce apoptotic cell death and to understand the subsequent mechanisms provides the foundations to predict and optimize NP-based photothermal therapy to treat cancer patients suffering from chemo- and radioresistance info:eu-repo/grantAgreement/ES/MINECO/MAT2011-26851-CO2-01 info:eu-repo/grantAgreement/ES/MINECO/MAT2011-26851-CO2-02 info:eu-repo/semantics/closedAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 13.334 2015 CHEMISTRY, MULTIDISCIPLINARY 8 / 162 = 0.049 2015 Q1 T1 MATERIALS SCIENCE, MULTIDISCIPLINARY 9 / 270 = 0.033 2015 Q1 T1 CHEMISTRY, PHYSICAL 6 / 144 = 0.042 2015 Q1 T1 NANOSCIENCE & NANOTECHNOLOGY 4 / 82 = 0.049 2015 Q1 T1 6.712 2015 Engineering (miscellaneous) 2015 Q1 Physics and Astronomy (miscellaneous) 2015 Q1 Nanoscience and Nanotechnology 2015 Q1 Materials Science (miscellaneous) 2015 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Del Pino, P. (orcid)0000-0002-1477-5259 Mitchell, S. G. (orcid)0000-0003-4848-414X Moros, M. (orcid)0000-0002-2861-2469 Stepien, G. (orcid)0000-0002-4170-7999 Pelaz, B. Parak, W. J. Gálvez, E. M. (orcid)0000-0001-6928-5516 Pardo, J. Universidad de Zaragoza (orcid)0000-0003-0154-0730 De La Fuente, J.M. Universidad de Zaragoza (orcid)0000-0003-1081-8482 1008 566 Universidad de Zaragoza Dpto. Microb.Med.Pr.,Sal.Públ. Área Inmunología 2013 765 Universidad de Zaragoza Dpto. Química Orgánica Área Química Orgánica 9, 1 (2015), 52-61 ACS Nano ACS NANO 1936-0851 632995 http://zaguan.unizar.es/record/147214/files/texto_completo.pdf Versión publicada 2742933 http://zaguan.unizar.es/record/147214/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:147214 articulos driver 2024-12-16-11:27:24 ARTICLE