000147809 001__ 147809
000147809 005__ 20250107161405.0
000147809 0247_ $$2doi$$a10.1128/JVI.01324-19
000147809 0248_ $$2sideral$$a115792
000147809 037__ $$aART-2019-115792
000147809 041__ $$aeng
000147809 100__ $$aNgo, M.H.
000147809 245__ $$aTracking the Fate of Endogenous Retrovirus Segregation in Wild and Domestic Cats
000147809 260__ $$c2019
000147809 5060_ $$aAccess copy available to the general public$$fUnrestricted
000147809 5203_ $$aEndogenous retroviruses (ERVs) of domestic cats (ERV-DCs) are one of the youngest feline ERV groups in domestic cats (Felis silvestris catus); some members are replication competent (ERV-DC10, ERV-DC18, and ERV-DC14), produce the antiretroviral soluble factor Refrex-1 (ERV-DC7 and ERV-DC16), or can generate recombinant feline leukemia virus (FeLV). Here, we investigated ERV-DC in European wildcats (Felis silvestris silvestris) and detected four loci: ERV-DC6, ERV-DC7, ERV-DC14, and ERV-DC16. ERV-DC14 was detected at a high frequency in European wildcats; however, it was replication defective due to a single G ¿ A nucleotide substitution, resulting in an E148K substitution in the ERV-DC14 envelope (Env). This mutation results in a cleavage-defective Env that is not incorporated into viral particles. Introduction of the same mutation into feline and murine infectious gammaretroviruses resulted in a similar Env dysfunction. Interestingly, the same mutation was found in an FeLV isolate from naturally occurring thymic lymphoma and a mouse ERV, suggesting a common mechanism of virus inactivation. Refrex-1 was present in European wildcats; however, ERV-DC16, but not ERV-DC7, was unfixed in European wildcats. Thus, Refrex-1 has had an antiviral role throughout the evolution of the genus Felis, predating cat exposure to feline retroviruses. ERV-DC sequence diversity was present across wild and domestic cats but was locus dependent. In conclusion, ERVs have evolved species-specific phenotypes through the interplay between ERVs and their hosts. The mechanism of viral inactivation may be similar irrespective of the evolutionary history of retroviruses. The tracking of ancestral retroviruses can shed light on their roles in pathogenesis and host-virus evolution.IMPORTANCE Domestic cats (Felis silvestris catus) were domesticated from wildcats approximately 9, 000¿years ago via close interaction between humans and cats. During cat evolution, various exogenous retroviruses infected different cat lineages and generated numerous ERVs in the host genome, some of which remain replication competent. Here, we detected several ERV-DC loci in Felis silvestris silvestris Notably, a species-specific single nucleotide polymorphism in the ERV-DC14 env gene, which results in a replication-defective product, is highly prevalent in European wildcats, unlike the replication-competent ERV-DC14 that is commonly present in domestic cats. The presence of the same lethal mutation in the env genes of both FeLV and murine ERV provides a common mechanism shared by endogenous and exogenous retroviruses by which ERVs can be inactivated after endogenization. The antiviral role of Refrex-1 predates cat exposure to feline retroviruses. The existence of two ERV-DC14 phenotypes provides a unique model for understanding both ERV fate and cat domestication. Copyright
000147809 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000147809 590__ $$a4.501$$b2019
000147809 591__ $$aVIROLOGY$$b8 / 37 = 0.216$$c2019$$dQ1$$eT1
000147809 592__ $$a2.406$$b2019
000147809 593__ $$aImmunology$$c2019$$dQ1
000147809 593__ $$aVirology$$c2019$$dQ1
000147809 593__ $$aMicrobiology$$c2019$$dQ1
000147809 593__ $$aInsect Science$$c2019$$dQ1
000147809 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000147809 700__ $$0(orcid)0000-0002-6770-3400$$aArnal, M.$$uUniversidad de Zaragoza
000147809 700__ $$aSumi, R.
000147809 700__ $$aKawasaki, J.
000147809 700__ $$aMiyake, A.
000147809 700__ $$aGrant, C.K.
000147809 700__ $$aOtoi, T.
000147809 700__ $$0(orcid)0000-0003-3289-4267$$aFernández de Luco, D.$$uUniversidad de Zaragoza
000147809 700__ $$aNishigaki, K.
000147809 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000147809 773__ $$g93, 24 (2019), [22 pp.]$$pJ. virol.$$tJournal of virology$$x0022-538X
000147809 8564_ $$s1542174$$uhttps://zaguan.unizar.es/record/147809/files/texto_completo.pdf$$yPostprint
000147809 8564_ $$s2294226$$uhttps://zaguan.unizar.es/record/147809/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000147809 909CO $$ooai:zaguan.unizar.es:147809$$particulos$$pdriver
000147809 951__ $$a2025-01-07-14:05:26
000147809 980__ $$aARTICLE