doi:10.1038/jid.2014.487engSandoval, JuanDíaz-Lagares, AngelSalgado, RocíoServitje, OctavioCliment, FinaOrtiz-Romero, Pablo L.Pérez-Ferriols, AmparoGarcia-Muret, Maria P.Estrach, TeresaGarcia, MarNonell, LaraEsteller, ManelPujol, Ramon M.Espinet, BlancaGallardo, FernandoMicroRNA expression profiling and DNA methylation signature for deregulated MicroRNA in cutaneous T-Cell LymphomaART-2015-129716MicroRNAs usually regulate gene expression negatively, and aberrant expression has been involved in the development of several types of cancers. Microarray profiling of microRNA expression was performed to define a microRNA signature in a series of mycosis fungoides tumor stage (MFt, n=21) and CD30+ primary cutaneous anaplastic large cell lymphoma (CD30+ cALCL, n=11) samples in comparison with inflammatory dermatoses (ID, n=5). Supervised clustering confirmed a distinctive microRNA profile for cutaneous T-cell lymphoma (CTCL) with respect to ID. A 40 microRNA signature was found in MFt including upregulated onco-microRNAs (miR-146a, miR-142-3p/5p, miR-21, miR-181a/b, and miR-155) and downregulated tumor-suppressor microRNAs (miR-200ab/429 cluster, miR-10b, miR-193b, miR-141/200c, and miR-23b/27b). Regarding CD30+ cALCL, 39 differentially expressed microRNAs were identified. Particularly, overexpression of miR-155, miR-21, or miR-142-3p/5p and downregulation of the miR-141/200c clusters were observed. DNA methylation in microRNA gene promoters, as expression regulatory mechanism for deregulated microRNAs, was analyzed using Infinium 450K array and approximately one-third of the differentially expressed microRNAs showed significant DNA methylation differences. Two different microRNA methylation signatures for MFt and CD30+ cALCL were found. Correlation analysis showed an inverse relationship for microRNA promoter methylation and microRNA expression. These results reveal a subgroup-specific epigenetically regulated microRNA signatures for MFt and CD30+ cALCL patients.2015http://zaguan.unizar.es/record/14781410.1038/jid.2014.487http://zaguan.unizar.es/record/147814oai:zaguan.unizar.es:147814JOURNAL OF INVESTIGATIVE DERMATOLOGY 135, 4 (2015), 1128-1137All rights reservedhttp://www.europeana.eu/rights/rr-f/info:eu-repo/semantics/closedAccess