147814 20250109150036.0 doi 10.1038/jid.2014.487 sideral 129716 ART-2015-129716 eng Sandoval, Juan MicroRNA expression profiling and DNA methylation signature for deregulated MicroRNA in cutaneous T-Cell Lymphoma 2015 MicroRNAs usually regulate gene expression negatively, and aberrant expression has been involved in the development of several types of cancers. Microarray profiling of microRNA expression was performed to define a microRNA signature in a series of mycosis fungoides tumor stage (MFt, n=21) and CD30+ primary cutaneous anaplastic large cell lymphoma (CD30+ cALCL, n=11) samples in comparison with inflammatory dermatoses (ID, n=5). Supervised clustering confirmed a distinctive microRNA profile for cutaneous T-cell lymphoma (CTCL) with respect to ID. A 40 microRNA signature was found in MFt including upregulated onco-microRNAs (miR-146a, miR-142-3p/5p, miR-21, miR-181a/b, and miR-155) and downregulated tumor-suppressor microRNAs (miR-200ab/429 cluster, miR-10b, miR-193b, miR-141/200c, and miR-23b/27b). Regarding CD30+ cALCL, 39 differentially expressed microRNAs were identified. Particularly, overexpression of miR-155, miR-21, or miR-142-3p/5p and downregulation of the miR-141/200c clusters were observed. DNA methylation in microRNA gene promoters, as expression regulatory mechanism for deregulated microRNAs, was analyzed using Infinium 450K array and approximately one-third of the differentially expressed microRNAs showed significant DNA methylation differences. Two different microRNA methylation signatures for MFt and CD30+ cALCL were found. Correlation analysis showed an inverse relationship for microRNA promoter methylation and microRNA expression. These results reveal a subgroup-specific epigenetically regulated microRNA signatures for MFt and CD30+ cALCL patients. info:eu-repo/semantics/closedAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 6.915 2015 DERMATOLOGY 1 / 61 = 0.016 2015 Q1 T1 2.66 2015 Biochemistry 2015 Q1 Molecular Biology 2015 Q1 Dermatology 2015 Q1 Cell Biology 2015 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Díaz-Lagares, Angel Salgado, Rocío Servitje, Octavio Climent, Fina Ortiz-Romero, Pablo L. Pérez-Ferriols, Amparo Garcia-Muret, Maria P. (orcid)0000-0003-2078-8205 Estrach, Teresa Garcia, Mar Nonell, Lara Esteller, Manel Pujol, Ramon M. Espinet, Blanca Gallardo, Fernando 135, 4 (2015), 1128-1137 J. invest. dermatol. JOURNAL OF INVESTIGATIVE DERMATOLOGY 0022-202X 3432155 http://zaguan.unizar.es/record/147814/files/texto_completo.pdf Versión publicada 3339478 http://zaguan.unizar.es/record/147814/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:147814 articulos driver 2025-01-09-14:59:11 ARTICLE