000147908 001__ 147908 000147908 005__ 20250923084439.0 000147908 0247_ $$2doi$$a10.3390/molecules29245935 000147908 0248_ $$2sideral$$a141432 000147908 037__ $$aART-2024-141432 000147908 041__ $$aeng 000147908 100__ $$aBertella, Anis 000147908 245__ $$aPhytochemical Study and In Vitro Antioxidant Activity of Helianthemum cinereum Along with Antitumor Activity of the Isolated trans-Tiliroside and Luteolin 4'-O-ß-Xyloside 000147908 260__ $$c2024 000147908 5060_ $$aAccess copy available to the general public$$fUnrestricted 000147908 5203_ $$aTwelve compounds (1–12), kaempferol (1), luteolin (2), luteolin 4′-O-β-xyloside (3), luteolin 4′-O-β-glucoside (4), quercetin 4′-O-β-xyloside (5), kaempferol-3-O-[6″-O-(E)-p-coumaroyl]-β-D-glucoside (trans-tiliroside) (6), protocatechuic acid (7), gallic acid (8), methyl gallate (9), ethyl gallate (10), shikimic acid-3-O-gallate (11), and 3,3′,4′-tri-O-methyl-ellagic acid 4-sulfate (12), were isolated and identified from the aerial parts of Helianthemum cinereum (Cav.) Pers (synonym: Helianthemum rubellum C. Presl. All compounds were isolated by applying different chromatographic procedures, such as silica gel, RP-18 and Sephadex LH-20 columns. The structures were elucidated by extensive spectroscopic methods, mainly nuclear magnetic resonance NMR 1D and 2D, and mass spectrometry, as well as by comparison with the reported spectroscopic data. The two organic extracts, ethyl acetate (EtOAc) and butanol (BuOH), were evaluated for their potent phenolic and flavonoid contents using the Folin–Ciocalteu and aluminum chloride colorimetric methods. Furthermore, the antioxidant activity of the two extracts was determined using the DPPH, FRAP, and ABTS methods. Pure trans-tiliroside (6), the main isolated compound, and luteolin 4′-O-β-xyloside (3) were evaluated for their antitumor activity against the lung cancer (A549), melanoma (A375) and pancreatic cancer (Mia PaCa-2 and Panc-1) cell lines by MTT assay. 000147908 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000147908 590__ $$a4.6$$b2024 000147908 592__ $$a0.865$$b2024 000147908 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b75 / 239 = 0.314$$c2024$$dQ2$$eT1 000147908 593__ $$aPharmaceutical Science$$c2024$$dQ1 000147908 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b82 / 319 = 0.257$$c2024$$dQ2$$eT1 000147908 593__ $$aOrganic Chemistry$$c2024$$dQ1 000147908 593__ $$aAnalytical Chemistry$$c2024$$dQ1 000147908 593__ $$aPhysical and Theoretical Chemistry$$c2024$$dQ1 000147908 593__ $$aChemistry (miscellaneous)$$c2024$$dQ1 000147908 593__ $$aMolecular Medicine$$c2024$$dQ2 000147908 593__ $$aDrug Discovery$$c2024$$dQ2 000147908 593__ $$aMedicine (miscellaneous)$$c2024$$dQ2 000147908 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000147908 700__ $$aSmadi, Abla 000147908 700__ $$aBenhabrou, Hakim 000147908 700__ $$aSalvador, Diana 000147908 700__ $$aWrona, Magdalena 000147908 700__ $$aOliveira, Helena 000147908 700__ $$aSidaoui, Abouamama 000147908 700__ $$aGavril-Luminita, Georgiana 000147908 700__ $$aPinto, Diana C. G. A. 000147908 700__ $$aOlewnik-Kruszkowska, Ewa 000147908 700__ $$0(orcid)0000-0003-2685-5739$$aNerín, Cristina$$uUniversidad de Zaragoza 000147908 700__ $$aSilva, Artur M. S. 000147908 700__ $$aBitam, Fatma 000147908 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica 000147908 773__ $$g29, 24 (2024), 5935 [18 pp.]$$pMolecules (Basel, Online)$$tMolecules$$x1420-3049 000147908 8564_ $$s1308505$$uhttps://zaguan.unizar.es/record/147908/files/texto_completo.pdf$$yVersión publicada 000147908 8564_ $$s2760469$$uhttps://zaguan.unizar.es/record/147908/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000147908 909CO $$ooai:zaguan.unizar.es:147908$$particulos$$pdriver 000147908 951__ $$a2025-09-22-14:49:10 000147908 980__ $$aARTICLE