147908 20260217205538.0 doi 10.3390/molecules29245935 sideral 141432 ART-2024-141432 eng Bertella, Anis Phytochemical Study and In Vitro Antioxidant Activity of Helianthemum cinereum Along with Antitumor Activity of the Isolated trans-Tiliroside and Luteolin 4'-O-ß-Xyloside 2024 Access copy available to the general public Unrestricted Twelve compounds (1–12), kaempferol (1), luteolin (2), luteolin 4′-O-β-xyloside (3), luteolin 4′-O-β-glucoside (4), quercetin 4′-O-β-xyloside (5), kaempferol-3-O-[6″-O-(E)-p-coumaroyl]-β-D-glucoside (trans-tiliroside) (6), protocatechuic acid (7), gallic acid (8), methyl gallate (9), ethyl gallate (10), shikimic acid-3-O-gallate (11), and 3,3′,4′-tri-O-methyl-ellagic acid 4-sulfate (12), were isolated and identified from the aerial parts of Helianthemum cinereum (Cav.) Pers (synonym: Helianthemum rubellum C. Presl. All compounds were isolated by applying different chromatographic procedures, such as silica gel, RP-18 and Sephadex LH-20 columns. The structures were elucidated by extensive spectroscopic methods, mainly nuclear magnetic resonance NMR 1D and 2D, and mass spectrometry, as well as by comparison with the reported spectroscopic data. The two organic extracts, ethyl acetate (EtOAc) and butanol (BuOH), were evaluated for their potent phenolic and flavonoid contents using the Folin–Ciocalteu and aluminum chloride colorimetric methods. Furthermore, the antioxidant activity of the two extracts was determined using the DPPH, FRAP, and ABTS methods. Pure trans-tiliroside (6), the main isolated compound, and luteolin 4′-O-β-xyloside (3) were evaluated for their antitumor activity against the lung cancer (A549), melanoma (A375) and pancreatic cancer (Mia PaCa-2 and Panc-1) cell lines by MTT assay. info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es 4.6 2024 CHEMISTRY, MULTIDISCIPLINARY 76 / 239 = 0.318 2024 Q2 T1 BIOCHEMISTRY & MOLECULAR BIOLOGY 82 / 320 = 0.256 2024 Q2 T1 0.865 2024 Pharmaceutical Science 2024 Q1 Organic Chemistry 2024 Q1 Analytical Chemistry 2024 Q1 Physical and Theoretical Chemistry 2024 Q1 Chemistry (miscellaneous) 2024 Q1 Molecular Medicine 2024 Q2 Drug Discovery 2024 Q2 Medicine (miscellaneous) 2024 Q2 8.6 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Smadi, Abla Benhabrou, Hakim Salvador, Diana Wrona, Magdalena Oliveira, Helena Sidaoui, Abouamama Gavril-Luminita, Georgiana Pinto, Diana C. G. A. Olewnik-Kruszkowska, Ewa Nerín, Cristina Universidad de Zaragoza (orcid)0000-0003-2685-5739 Silva, Artur M. S. Bitam, Fatma 2009 750 Universidad de Zaragoza Dpto. Química Analítica Área Química Analítica 29, 24 (2024), 5935 [18 pp.] Molecules (Basel, Online) Molecules 1420-3049 1308505 http://zaguan.unizar.es/record/147908/files/texto_completo.pdf Versión publicada 2760469 http://zaguan.unizar.es/record/147908/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:147908 articulos driver 2026-02-17-20:35:19 ARTICLE