000147928 001__ 147928 000147928 005__ 20260112133316.0 000147928 0247_ $$2doi$$a10.3390/agriculture14122350 000147928 0248_ $$2sideral$$a141431 000147928 037__ $$aART-2024-141431 000147928 041__ $$aeng 000147928 100__ $$0(orcid)0000-0001-5635-413X$$aTejedor-Calvo, Eva$$uUniversidad de Zaragoza 000147928 245__ $$aMass Spectrometry-Based Non-Targeted Lipidome Analysis and Extraction of Markers for the Authentication of White and Black Truffle Species and Their Origin Determination 000147928 260__ $$c2024 000147928 5060_ $$aAccess copy available to the general public$$fUnrestricted 000147928 5203_ $$aThe visual authentication of high-value truffles (Tuber magnatum and Tuber melanosporum) is challenging, as they share similar morphological characteristics with other truffle species that have a lower economic value. This similarity complicates accurate identification and increases the risk of substitution or mislabeling, which can affect both market prices and consumer trust. For this reason, the aim of this study was to apply a non-targeted lipidomic approach using ion mobility spectrometry-mass spectrometry to distinguish between white (T. magnatum, Tuber borchii, and Tuber oligospermum) and black truffle species (T. melanosprum, T. aestivum, T. aestivum var. uncinatum, T. brumale, and T. indicum) and to determine the different geographical origins of the two most valuable truffle species (T. melanosporum and T. magnatum). Among several hundred features, 37 and 57 lipids were identified as marker compounds to distinguish white and black truffle species using MS/MS spectra and collision cross section (CCS) values, respectively. Only a few marker compounds were necessary to recognize the differences between white and black truffles. In particular, ceramides, glycerolipids, and phospholipids proved to be particularly suitable for separating the species. In addition, different metabolite profiles were determined for T. melanosporum and T. magnatum depending on their geographical origin. These findings lay the groundwork for a comprehensive quality control framework for fresh truffles, ensuring authenticity, detecting adulteration, and preserving their premium status. 000147928 536__ $$9info:eu-repo/grantAgreement/EC/H2020/101007623/EU/INnovation in Truffle cultivation, preservAtion, proCessing and wild truffle resources managemenT/INTACT$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 101007623-INTACT$$9info:eu-repo/grantAgreement/ES/MICINN/JDC2022-048252-I 000147928 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es 000147928 590__ $$a3.6$$b2024 000147928 592__ $$a0.704$$b2024 000147928 591__ $$aAGRONOMY$$b19 / 129 = 0.147$$c2024$$dQ1$$eT1 000147928 593__ $$aAgronomy and Crop Science$$c2024$$dQ1 000147928 593__ $$aPlant Science$$c2024$$dQ1 000147928 593__ $$aFood Science$$c2024$$dQ2 000147928 594__ $$a6.3$$b2024 000147928 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000147928 700__ $$0(orcid)0000-0003-3384-7534$$aMarco, Pedro$$uUniversidad de Zaragoza 000147928 700__ $$aFischer, Markus 000147928 700__ $$aCreydt, Marina 000147928 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica 000147928 7102_ $$12008$$2640$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Nutrición Bromatología 000147928 773__ $$g14, 12 (2024), 2350 [20 pp.]$$pAgriculture (Basel)$$tAgriculture (Basel)$$x2077-0472 000147928 8564_ $$s8419748$$uhttps://zaguan.unizar.es/record/147928/files/texto_completo.pdf$$yVersión publicada 000147928 8564_ $$s2849485$$uhttps://zaguan.unizar.es/record/147928/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000147928 909CO $$ooai:zaguan.unizar.es:147928$$particulos$$pdriver 000147928 951__ $$a2026-01-12-13:04:27 000147928 980__ $$aARTICLE