000148173 001__ 148173
000148173 005__ 20251219150007.0
000148173 0247_ $$2doi$$a10.1177/0300985816641993
000148173 0248_ $$2sideral$$a96620
000148173 037__ $$aART-2016-96620
000148173 041__ $$aeng
000148173 100__ $$0(orcid)0000-0002-0680-9957$$aBorobia, M.
000148173 245__ $$aJaagsiekte Sheep Retrovirus Can Reach Peyer’s Patches and Mesenteric Lymph Nodes of Lambs Nursed by Infected Mothers
000148173 260__ $$c2016
000148173 5060_ $$aAccess copy available to the general public$$fUnrestricted
000148173 5203_ $$aOvine pulmonary adenocarcinoma (OPA) is a contagious lung cancer of sheep caused by jaagsiekte sheep retrovirus (JSRV). It is generally accepted that transmission by the respiratory route occurs under natural conditions. However recent studies strongly indicate that JSRV can also be transmitted to lambs perinatally via colostrum and milk (C/M). The aim of this work was to confirm that C/M can transmit JSRV infection to lambs under natural conditions and investigate the initial events associated with this transmission route. We have analyzed the presence of JSRV in C/M samples from 22 naturally infected, asymptomatic ewes throughout a lactation period, and in various tissues collected from a group of 36 of their lambs that were fed naturally. The lambs were euthanized at 12, 24, 48, and 72 hours and at 5 and 10 days after birth. We detected JSRV-provirus by PCR in the somatic C/M cells from 10/22 ewes (45.45%). The virus was also detected in 9/36 lambs (25%). JSRV-infected cells, with lymphoreticular-like morphology, were observed by immunohistochemistry (IHC) and in situ hybridization (ISH) in Peyer’s patches (PP) from the small intestine of the youngest lambs and in mesenteric lymph nodes (MLN) from lambs older than 72 hours. The virus was also detected by PCR in white blood cells (WBC) in 2/36 lambs (5.5%). These results confirm colostral transmission of JSRV to lambs under natural conditions. Infected lymphoreticular cells contained in C/M appear to be involved. These cells can cross the intestinal barrier of newborn lambs, reach the MLN and enter into circulation.
000148173 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B103-09$$9info:eu-repo/grantAgreement/ES/MICINN/AGL2008-00803
000148173 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000148173 590__ $$a1.996$$b2016
000148173 591__ $$aVETERINARY SCIENCES$$b13 / 135 = 0.096$$c2016$$dQ1$$eT1
000148173 591__ $$aPATHOLOGY$$b37 / 78 = 0.474$$c2016$$dQ2$$eT2
000148173 592__ $$a0.937$$b2016
000148173 593__ $$aVeterinary (miscellaneous)$$c2016$$dQ1
000148173 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000148173 700__ $$0(orcid)0000-0003-3792-287X$$aDe las Heras, M.$$uUniversidad de Zaragoza
000148173 700__ $$0(orcid)0000-0002-2724-2785$$aRamos, J. J.$$uUniversidad de Zaragoza
000148173 700__ $$0(orcid)0000-0003-0042-8800$$aFerrer, L. M.$$uUniversidad de Zaragoza
000148173 700__ $$0(orcid)0000-0002-7822-6646$$aLacasta, D.$$uUniversidad de Zaragoza
000148173 700__ $$aDe Martino, A.
000148173 700__ $$0(orcid)0000-0002-2557-4890$$aFernández, A.$$uUniversidad de Zaragoza
000148173 700__ $$0(orcid)0000-0002-1934-5397$$aLoste, A.$$uUniversidad de Zaragoza
000148173 700__ $$aMarteles, D.
000148173 700__ $$0(orcid)0000-0002-0313-5926$$aOrtín, A.$$uUniversidad de Zaragoza
000148173 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000148173 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000148173 773__ $$g53, 6 (2016), 1172-1179$$pVet. pathol.$$tVeterinary Pathology$$x0300-9858
000148173 8564_ $$s522899$$uhttps://zaguan.unizar.es/record/148173/files/texto_completo.pdf$$yVersión publicada
000148173 8564_ $$s2543375$$uhttps://zaguan.unizar.es/record/148173/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000148173 909CO $$ooai:zaguan.unizar.es:148173$$particulos$$pdriver
000148173 951__ $$a2025-12-19-14:58:08
000148173 980__ $$aARTICLE