000148192 001__ 148192
000148192 005__ 20250114175434.0
000148192 0247_ $$2doi$$a10.1038/s41598-017-16134-6
000148192 0248_ $$2sideral$$a135602
000148192 037__ $$aART-2017-135602
000148192 041__ $$aeng
000148192 100__ $$aGurruchaga, Haritz
000148192 245__ $$aCryopreservation of Human Mesenchymal Stem Cells in an Allogeneic Bioscaffold based on Platelet Rich Plasma and Synovial Fluid
000148192 260__ $$c2017
000148192 5060_ $$aAccess copy available to the general public$$fUnrestricted
000148192 5203_ $$aTransplantation of mesenchymal stem cells (MSCs) has emerged as an alternative strategy to treat knee osteoarthritis. In this context, MSCs derived from synovial fluid could provide higher chondrogenic and cartilage regeneration, presenting synovial fluid as an appropriate MSCs source. An allogeneic and biomimetic bioscaffold composed of Platelet Rich Plasma and synovial fluid that preserve and mimics the natural environment of MSCs isolated from knee has also been developed. We have optimized the cryopreservation of knee-isolated MSCs embedded within the aforementioned biomimetic scaffold, in order to create a reserve of young autologous embedded knee MSCs for future clinical applications. We have tested several cryoprotectant solutions combining dimethyl sulfoxide (DMSO), sucrose and human serum and quantifying the viability and functionality of the embedded MSCs after thawing. MSCs embedded in bioscaffolds cryopreserved with DMSO 10% or the combination of DMSO 10% and Sucrose 0,2 M displayed the best cell viabilities maintaining the multilineage differentiation potential of MSCs after thawing. In conclusion, embedded young MSCs within allogeneic biomimetic bioscaffold can be cryopreserved with the cryoprotectant solutions described in this work, allowing their future clinical use in patients with cartilage defects.
000148192 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000148192 590__ $$a4.122$$b2017
000148192 591__ $$aMULTIDISCIPLINARY SCIENCES$$b11 / 63 = 0.175$$c2017$$dQ1$$eT1
000148192 592__ $$a1.533$$b2017
000148192 593__ $$aMultidisciplinary$$c2017$$dQ1
000148192 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000148192 700__ $$aSaenz del Burgo, Laura
000148192 700__ $$aGarate, Ane
000148192 700__ $$aDelgado, Diego
000148192 700__ $$aSanchez, Pello
000148192 700__ $$aOrive, Gorka
000148192 700__ $$0(orcid)0000-0002-8666-622X$$aCiriza, Jesús
000148192 700__ $$aSanchez, Mikel
000148192 700__ $$aPedraz, Jose Luis
000148192 773__ $$g7, 1 (2017), 15733 [12 pp.]$$pSci. rep. (Nat. Publ. Group)$$tScientific reports (Nature Publishing Group)$$x2045-2322
000148192 8564_ $$s3906655$$uhttps://zaguan.unizar.es/record/148192/files/texto_completo.pdf$$yVersión publicada
000148192 8564_ $$s2589991$$uhttps://zaguan.unizar.es/record/148192/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000148192 909CO $$ooai:zaguan.unizar.es:148192$$particulos$$pdriver
000148192 951__ $$a2025-01-14-15:49:13
000148192 980__ $$aARTICLE