000148268 001__ 148268
000148268 005__ 20250115160155.0
000148268 0247_ $$2doi$$a10.1177/10406387211039196
000148268 0248_ $$2sideral$$a124783
000148268 037__ $$aART-2021-124783
000148268 041__ $$aeng
000148268 100__ $$0(orcid)0000-0002-0680-9957$$aBorobia, Marta$$uUniversidad de Zaragoza
000148268 245__ $$aJaagsiekte sheep retrovirus found in milk macrophages but not in milk lymphocytes or mammary gland epithelia of naturally infected sheep
000148268 260__ $$c2021
000148268 5060_ $$aAccess copy available to the general public$$fUnrestricted
000148268 5203_ $$aJaagsiekte sheep retrovirus (JSRV) causes ovine pulmonary adenocarcinoma. JSRV can be transmitted via infected colostrum or milk, which contain somatic cells (SCs) harboring JSRV provirus. Nevertheless, the cell types involved in this form of transmission and the involvement of the mammary gland remain unknown. We separated adherent cells (macrophages and monocytes) by plastic adherence, and lymphocytes (CD4+ and CD8+ T cells, and B cells) by flow cytometry, from SCs in milk samples from 12 naturally infected, PCR blood test JSRV-positive, subclinical ewes. These cell populations were tested by PCR to detect JSRV provirus. The ewes were euthanized, and mammary gland samples were analyzed immunohistochemically to detect JSRV surface protein. We did not detect JSRV provirus in any milk lymphocyte population, but milk adherent cells were positive in 3 of 12 sheep, suggesting a potential major role of this population in the lactogenic transmission of JSRV. Immunohistochemistry did not reveal positive results in mammary epithelial cells, pointing to a lack of participation of the mammary gland in the biological cycle of JSRV and reducing the probability of excretion of free viral particles in colostrum or milk.
000148268 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/AGL2008-00803
000148268 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000148268 590__ $$a1.569$$b2021
000148268 591__ $$aVETERINARY SCIENCES$$b74 / 145 = 0.51$$c2021$$dQ3$$eT2
000148268 592__ $$a0.457$$b2021
000148268 593__ $$aVeterinary (miscellaneous)$$c2021$$dQ2
000148268 594__ $$a2.3$$b2021
000148268 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000148268 700__ $$0(orcid)0000-0003-3792-287X$$aDe las Heras, Marcelo$$uUniversidad de Zaragoza
000148268 700__ $$aGodino, Javier
000148268 700__ $$0(orcid)0000-0003-0042-8800$$aFerrer, Luis M.$$uUniversidad de Zaragoza
000148268 700__ $$0(orcid)0000-0002-7822-6646$$aLacasta, Delia$$uUniversidad de Zaragoza
000148268 700__ $$0(orcid)0000-0002-1934-5397$$aLoste, Araceli$$uUniversidad de Zaragoza
000148268 700__ $$0(orcid)0000-0002-2724-2785$$aRamos, Juan J.$$uUniversidad de Zaragoza
000148268 700__ $$0(orcid)0000-0002-0313-5926$$aOrtín, Aurora$$uUniversidad de Zaragoza
000148268 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000148268 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000148268 773__ $$g78 (2021), 104063872110391$$pJ. vet. diagn. invest.$$tJOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION$$x1040-6387
000148268 8564_ $$s482881$$uhttps://zaguan.unizar.es/record/148268/files/texto_completo.pdf$$yVersión publicada
000148268 8564_ $$s2913497$$uhttps://zaguan.unizar.es/record/148268/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000148268 909CO $$ooai:zaguan.unizar.es:148268$$particulos$$pdriver
000148268 951__ $$a2025-01-15-15:06:07
000148268 980__ $$aARTICLE