148268 20250115160155.0 doi 10.1177/10406387211039196 sideral 124783 ART-2021-124783 eng Borobia, Marta Universidad de Zaragoza (orcid)0000-0002-0680-9957 Jaagsiekte sheep retrovirus found in milk macrophages but not in milk lymphocytes or mammary gland epithelia of naturally infected sheep 2021 Jaagsiekte sheep retrovirus (JSRV) causes ovine pulmonary adenocarcinoma. JSRV can be transmitted via infected colostrum or milk, which contain somatic cells (SCs) harboring JSRV provirus. Nevertheless, the cell types involved in this form of transmission and the involvement of the mammary gland remain unknown. We separated adherent cells (macrophages and monocytes) by plastic adherence, and lymphocytes (CD4+ and CD8+ T cells, and B cells) by flow cytometry, from SCs in milk samples from 12 naturally infected, PCR blood test JSRV-positive, subclinical ewes. These cell populations were tested by PCR to detect JSRV provirus. The ewes were euthanized, and mammary gland samples were analyzed immunohistochemically to detect JSRV surface protein. We did not detect JSRV provirus in any milk lymphocyte population, but milk adherent cells were positive in 3 of 12 sheep, suggesting a potential major role of this population in the lactogenic transmission of JSRV. Immunohistochemistry did not reveal positive results in mammary epithelial cells, pointing to a lack of participation of the mammary gland in the biological cycle of JSRV and reducing the probability of excretion of free viral particles in colostrum or milk. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/ES/MICINN/AGL2008-00803 info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 1.569 2021 VETERINARY SCIENCES 74 / 145 = 0.51 2021 Q3 T2 0.457 2021 Veterinary (miscellaneous) 2021 Q2 2.3 2021 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion De las Heras, Marcelo Universidad de Zaragoza (orcid)0000-0003-3792-287X Godino, Javier Ferrer, Luis M. Universidad de Zaragoza (orcid)0000-0003-0042-8800 Lacasta, Delia Universidad de Zaragoza (orcid)0000-0002-7822-6646 Loste, Araceli Universidad de Zaragoza (orcid)0000-0002-1934-5397 Ramos, Juan J. Universidad de Zaragoza (orcid)0000-0002-2724-2785 Ortín, Aurora Universidad de Zaragoza (orcid)0000-0002-0313-5926 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 78 (2021), 104063872110391 J. vet. diagn. invest. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION 1040-6387 482881 http://zaguan.unizar.es/record/148268/files/texto_completo.pdf Versión publicada 2913497 http://zaguan.unizar.es/record/148268/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:148268 articulos driver 2025-01-15-15:06:07 ARTICLE