000148337 001__ 148337
000148337 005__ 20250116161254.0
000148337 0247_ $$2doi$$a10.1039/c6dt02878k
000148337 0248_ $$2sideral$$a106283
000148337 037__ $$aART-2016-106283
000148337 041__ $$aeng
000148337 100__ $$0(orcid)0000-0002-1753-5103$$aVisbal, Renso
000148337 245__ $$aCytotoxicity and biodistribution studies of luminescent Au(I) and Ag(I) N-heterocyclic carbenes. Searching for new biological targets
000148337 260__ $$c2016
000148337 5060_ $$aAccess copy available to the general public$$fUnrestricted
000148337 5203_ $$aA range of fluorescent and biologically compatible gold(I)-N-heterocyclic carbenes bearing acridine as a wingtip group and either a 2-mercaptopyridine or a tetra-O-acetyl-1-thio-beta-D-glucopyranoside as an ancillary ligand has been synthesised. Their luminescence, cytotoxicity and biodistribution have been investigated together with those of analogous gold(I) and silver(I) chloride-and bis-NHC complexes. All complexes displayed emissions based on IL transitions centred on the acridine moiety. The cytotoxic activity measured in lung, A549, and pancreatic, MiaPaca2, carcinoma cell lines revealed a general cytotoxicity pattern (thiolate > biscarbene > chloride derivatives) and flow cytometry assays pointed towards apoptosis as the cell death mechanism. Moreover, fluorescence cell microscopy disclosed an unusual biodistribution behavior, being mainly localised in lysosomes and to a lesser extent in the nucleus. Preliminary DNA interaction experiments suggested the metal fragment and not the acridine moiety as responsible for such biodistribution, which widen the scope for new biological targets.
000148337 536__ $$9info:eu-repo/grantAgreement/ES/DGA-FSE/E77$$9info:eu-repo/grantAgreement/ES/MICINN/SAF2013-48626-C2-2-R$$9info:eu-repo/grantAgreement/ES/MINECO/CTQ2015-70371-REDT$$9info:eu-repo/grantAgreement/ES/MINECO-FEDER/CTQ2013-48635-C2-1-P
000148337 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000148337 590__ $$a4.029$$b2016
000148337 591__ $$aCHEMISTRY, INORGANIC & NUCLEAR$$b7 / 46 = 0.152$$c2016$$dQ1$$eT1
000148337 592__ $$a1.229$$b2016
000148337 593__ $$aInorganic Chemistry$$c2016$$dQ1
000148337 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000148337 700__ $$0(orcid)0000-0002-1218-7218$$aFernández-Moreira, Vanesa
000148337 700__ $$0(orcid)0000-0002-2315-9079$$aMarzo, Isabel$$uUniversidad de Zaragoza
000148337 700__ $$0(orcid)0000-0003-1984-7538$$aLaguna, Antonio$$uUniversidad de Zaragoza
000148337 700__ $$0(orcid)0000-0003-0553-0695$$aGimeno, M.Concepción$$uUniversidad de Zaragoza
000148337 7102_ $$12010$$2760$$aUniversidad de Zaragoza$$bDpto. Química Inorgánica$$cÁrea Química Inorgánica
000148337 7102_ $$11002$$2050$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Biología Celular
000148337 773__ $$g45, 38 (2016), 15026-15033$$pDalton Trans.$$tDalton Transactions$$x1477-9226
000148337 8564_ $$s1027532$$uhttps://zaguan.unizar.es/record/148337/files/texto_completo.pdf$$yPostprint
000148337 8564_ $$s2858814$$uhttps://zaguan.unizar.es/record/148337/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000148337 909CO $$ooai:zaguan.unizar.es:148337$$particulos$$pdriver
000148337 951__ $$a2025-01-16-14:31:32
000148337 980__ $$aARTICLE