148337 20250116161254.0 doi 10.1039/c6dt02878k sideral 106283 ART-2016-106283 eng Visbal, Renso (orcid)0000-0002-1753-5103 Cytotoxicity and biodistribution studies of luminescent Au(I) and Ag(I) N-heterocyclic carbenes. Searching for new biological targets 2016 A range of fluorescent and biologically compatible gold(I)-N-heterocyclic carbenes bearing acridine as a wingtip group and either a 2-mercaptopyridine or a tetra-O-acetyl-1-thio-beta-D-glucopyranoside as an ancillary ligand has been synthesised. Their luminescence, cytotoxicity and biodistribution have been investigated together with those of analogous gold(I) and silver(I) chloride-and bis-NHC complexes. All complexes displayed emissions based on IL transitions centred on the acridine moiety. The cytotoxic activity measured in lung, A549, and pancreatic, MiaPaca2, carcinoma cell lines revealed a general cytotoxicity pattern (thiolate > biscarbene > chloride derivatives) and flow cytometry assays pointed towards apoptosis as the cell death mechanism. Moreover, fluorescence cell microscopy disclosed an unusual biodistribution behavior, being mainly localised in lysosomes and to a lesser extent in the nucleus. Preliminary DNA interaction experiments suggested the metal fragment and not the acridine moiety as responsible for such biodistribution, which widen the scope for new biological targets. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/ES/DGA-FSE/E77 info:eu-repo/grantAgreement/ES/MICINN/SAF2013-48626-C2-2-R info:eu-repo/grantAgreement/ES/MINECO/CTQ2015-70371-REDT info:eu-repo/grantAgreement/ES/MINECO-FEDER/CTQ2013-48635-C2-1-P info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 4.029 2016 CHEMISTRY, INORGANIC & NUCLEAR 7 / 46 = 0.152 2016 Q1 T1 1.229 2016 Inorganic Chemistry 2016 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Fernández-Moreira, Vanesa (orcid)0000-0002-1218-7218 Marzo, Isabel Universidad de Zaragoza (orcid)0000-0002-2315-9079 Laguna, Antonio Universidad de Zaragoza (orcid)0000-0003-1984-7538 Gimeno, M.Concepción Universidad de Zaragoza (orcid)0000-0003-0553-0695 2010 760 Universidad de Zaragoza Dpto. Química Inorgánica Área Química Inorgánica 1002 050 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Biología Celular 45, 38 (2016), 15026-15033 Dalton Trans. Dalton Transactions 1477-9226 1027532 http://zaguan.unizar.es/record/148337/files/texto_completo.pdf Postprint 2858814 http://zaguan.unizar.es/record/148337/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:148337 articulos driver 2025-01-16-14:31:32 ARTICLE