000148369 001__ 148369
000148369 005__ 20250116161254.0
000148369 0247_ $$2doi$$a10.1183/13993003.01401-2022
000148369 0248_ $$2sideral$$a133289
000148369 037__ $$aART-2023-133289
000148369 041__ $$aeng
000148369 100__ $$aDíaz-García, E.
000148369 245__ $$aInflammasome activation mediated by oxidised low-density lipoprotein in patients with sleep apnoea and early subclinical atherosclerosis
000148369 260__ $$c2023
000148369 5203_ $$aAtherosclerosis is a common comorbidity of obstructive sleep apnoea (OSA) patients, caused by the interaction of dyslipidaemia and systemic inflammation. The OSA pro-inflammatory response is mediated by NLRP3 inflammasome activation, which requires a priming signal mediated by intermittent hypoxia (IH) and an activation signal provided by soluble stimulus present in plasma. Our objectives were to study oxidised low-density lipoprotein (oxLDL) expression in OSA patients with or without early subclinical atherosclerosis (eSA) as well as its contribution to NLRP3 activation and tissue factor (TF) release.MethodsWe analysed oxLDL, key components of the NLRP3 inflammasome cascade and TF in plasma and monocytes from OSA patients and non-apnoeic subjects, with or without eSA as determined by increased carotid intima–media thickness without the appearance of atherosclerotic plaques. The oxLDL contribution to NLRP3 inflammasome activation was assessed usingin vitromodels.ResultsHigh levels of oxLDL were identified in plasma from OSA patients, particularly in those with eSA, as well as an overexpression of NLRP3 cascade components and TF. Furthermore,in vitromodels showed that both oxLDL and plasma from OSA patients with eSA act synergistically with IH as a priming and activation signal of NLRP3 that enhances the inflammatory response, pyroptosis and TF release.ConclusionsOSA patients with eSA exhibit NLRP3 activation by IH and the presence of oxLDL capable of releasing TF, constituting a pathway for the interaction between dyslipidaemia and systemic inflammation in the development of atherosclerotic lesions.
000148369 536__ $$9info:eu-repo/grantAgreement/ES/FIS/PI13-01512$$9info:eu-repo/grantAgreement/ES/FIS/PI15-01949$$9info:eu-repo/grantAgreement/ES/FIS/PI16-00201$$9info:eu-repo/grantAgreement/ES/FIS/PI18-00028$$9info:eu-repo/grantAgreement/ES/FIS/PI18-01363$$9info:eu-repo/grantAgreement/ES/FIS/PI19-01612$$9info:eu-repo/grantAgreement/ES/ISCIII-FEDER-FIS/PI18-01524$$9info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI12-02175
000148369 540__ $$9info:eu-repo/semantics/closedAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000148369 590__ $$a17.0$$b2023
000148369 591__ $$aRESPIRATORY SYSTEM$$b4 / 101 = 0.04$$c2023$$dQ1$$eT1
000148369 592__ $$a3.81$$b2023
000148369 593__ $$aPulmonary and Respiratory Medicine$$c2023$$dQ1
000148369 593__ $$aMedicine (miscellaneous)$$c2023$$dQ1
000148369 594__ $$a27.5$$b2023
000148369 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000148369 700__ $$0(orcid)0000-0002-5228-248X$$aSanz-Rubio, D.
000148369 700__ $$aGarcía-Tovar, S.
000148369 700__ $$aAlfaro, E.
000148369 700__ $$aCubero, P.
000148369 700__ $$aGil, A. V.
000148369 700__ $$0(orcid)0000-0001-9096-2294$$aMarín, J. M.$$uUniversidad de Zaragoza
000148369 700__ $$aCubillos-Zapata, C.
000148369 700__ $$aGarcía-Río, F.
000148369 7102_ $$11007$$2610$$aUniversidad de Zaragoza$$bDpto. Medicina, Psiqu. y Derm.$$cArea Medicina
000148369 773__ $$g61, 3 (2023), 2201401 [33 pp.]$$pEur. respir. j.$$tEuropean respiratory journal$$x0903-1936
000148369 8564_ $$s825111$$uhttps://zaguan.unizar.es/record/148369/files/texto_completo.pdf$$yVersión publicada
000148369 8564_ $$s2696092$$uhttps://zaguan.unizar.es/record/148369/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000148369 909CO $$ooai:zaguan.unizar.es:148369$$particulos$$pdriver
000148369 951__ $$a2025-01-16-14:32:08
000148369 980__ $$aARTICLE