148444 20250121144846.0 doi 10.1016/j.foodres.2020.109054 sideral 116564 ART-2020-116564 eng Tejedor-Calvo, E. (orcid)0000-0001-5635-413X Screening of bioactive compounds in truffles and evaluation of pressurized liquid extractions (PLE) to obtain fractions with biological activities 2020 Access copy available to the general public Unrestricted Truffles, besides the appreciated aromatic compounds, contain other molecules with interesting bioactive properties. A screening of fungal sterols and ß-glucans within different truffle species and locations was carried out. These compounds were extracted with pressurized liquids (PLE) generating enriched fractions. Extraction efficiency was studied with a full-factorial experimental design (Response surface methodology, RSM), using water and ethanol as extraction solvents. Polysaccharides from truffle powder (TP) and the optimal PLE extract (EP) obtained were precipitated and analysed by NMR and GC–MS. THP-1 cell cultures were utilized to test immunomodulatory properties. With the optimal PLE conditions (16.7 MPa, 180 °C, 30 min) 64 and 22.5% yields were obtained respect, with water and ethanol, generating fractions containing respect, 9.1% ß-glucan and 4.5% ergosterol. NMR analyses detected (1 ¿ 3)-ß-glucan structures in truffle. The EP induced a reduction of 40% IL-1ß and 60% IL-6 pro-inflammatory cytokines secretion suggesting potential immunomodulatory activity. © 2020 Elsevier Ltd info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 6.475 2020 FOOD SCIENCE & TECHNOLOGY 9 / 143 = 0.063 2020 Q1 T1 1.479 2020 Food Science 2020 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Morales, D. Marco, P. Universidad de Zaragoza (orcid)0000-0003-3384-7534 Sánchez, S. Garcia-Barreda, S. Universidad de Zaragoza (orcid)0000-0002-7248-234X Smiderle, F. R. Iacomini, M. Villalva, M. Santoyo, S. Soler-Rivas, C. 2008 640 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Nutrición Bromatología 5011 500 Universidad de Zaragoza Dpto. CC.Agrar.y Medio Natural Area Ingeniería Agroforestal 132 (2020), 109054 [8 pp.] Food res. int. Food Research International 0963-9969 511610 http://zaguan.unizar.es/record/148444/files/texto_completo.pdf Postprint 1132760 http://zaguan.unizar.es/record/148444/files/texto_completo.jpg?subformat=icon icon Postprint https://www.scopus.com/inward/record.uri?eid=2-s2.0-85079197143&doi=10.1016%2fj.foodres.2020.109054&partnerID=40&md5=bc28d978aca68efac91e51b4026a9a20 Texto completo de la revista oai:zaguan.unizar.es:148444 articulos driver 2025-01-21-14:47:40 ARTICLE