000148483 001__ 148483
000148483 005__ 20250117162508.0
000148483 0247_ $$2doi$$a10.3390/ani15010083
000148483 0248_ $$2sideral$$a141867
000148483 037__ $$aART-2025-141867
000148483 041__ $$aeng
000148483 100__ $$aDoghbri, Lamia
000148483 245__ $$aMelatonin in Male Dromedary Camel (Camelus dromedarius) Seminal Plasma and Its Specific MT1 and MT2 Receptors on Sperm Membranes
000148483 260__ $$c2025
000148483 5060_ $$aAccess copy available to the general public$$fUnrestricted
000148483 5203_ $$aCamels (Camelus dromedarius) are seasonal short-day breeders, regulated by photoperiod and melatonin secretion. However, no studies have explored melatonin levels in camel seminal plasma or their relationship with testosterone, age, or climatic factors, nor is it known whether melatonin receptors exist in camel spermatozoa to respond to seminal melatonin. This study aimed to analyze melatonin levels in camel seminal plasma and its specific receptors in spermatozoa. Semen samples were obtained from November to March (breeding season). Testosterone and melatonin levels were measured in seminal plasma by ELISA. Melatonin receptors were localized in spermatozoa using immunofluorescence, and their presence was confirmed by Western Blot. Melatonin levels were higher from November to January and decreased in February and March. No correlation between testosterone and melatonin levels was found, but both hormones were negatively correlated with daylength (p = 0.0089 and p = 0.0688, respectively). Testosterone, but not melatonin, levels were affected by age. Two melatonin receptors (MT1, MT2) were detected on camel spermatozoa, with several immunotypes labeled mainly in the tail and post-acrosome region, but also in the acrosome and neck. Western Blot analysis confirmed the presence of these receptors, showing a 39 kDa band for MT1 and a 36 kDa band for MT2. Understanding melatonin’s effects on sperm could help ejaculates’ processing procedures, semen handling, and infertility issues in camels.
000148483 536__ $$9info:eu-repo/grantAgreement/ES/CICYT-FEDER/AGL2017-83799-R$$9info:eu-repo/grantAgreement/ES/DGA/A07-17R
000148483 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000148483 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000148483 700__ $$0(orcid)0000-0003-3929-5064$$aCarvajal-Serna, Melissa
000148483 700__ $$aAtigui, Moufida
000148483 700__ $$0(orcid)0000-0003-1997-4262$$aCasao, Adriana$$uUniversidad de Zaragoza
000148483 700__ $$0(orcid)0000-0002-4670-3735$$aPeña-Delgado, Victoria
000148483 700__ $$aSeddik, Mabrouk-Mouldi
000148483 700__ $$aDbara, Mohamed
000148483 700__ $$0(orcid)0000-0002-2312-6402$$aPérez-Pé, Rosaura$$uUniversidad de Zaragoza
000148483 700__ $$aHammadi, Mohamed
000148483 7102_ $$11002$$2819$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Zoología
000148483 773__ $$g15, 1 (2025), 83 [17 pp.]$$pAnimals (Basel)$$tAnimals$$x2076-2615
000148483 8564_ $$s3318594$$uhttps://zaguan.unizar.es/record/148483/files/texto_completo.pdf$$yVersión publicada
000148483 8564_ $$s2729483$$uhttps://zaguan.unizar.es/record/148483/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000148483 909CO $$ooai:zaguan.unizar.es:148483$$particulos$$pdriver
000148483 951__ $$a2025-01-17-14:36:40
000148483 980__ $$aARTICLE