000148530 001__ 148530
000148530 005__ 20250121144845.0
000148530 0247_ $$2doi$$a10.1016/j.vetimm.2015.04.004
000148530 0248_ $$2sideral$$a90474
000148530 037__ $$aART-2015-90474
000148530 041__ $$aeng
000148530 100__ $$0(orcid)0000-0002-1075-8267$$aRemacha, A.R.$$uUniversidad de Zaragoza
000148530 245__ $$aExpression of genes involved in immune response and in vitro immunosuppressive effect of equine MSCs
000148530 260__ $$c2015
000148530 5060_ $$aAccess copy available to the general public$$fUnrestricted
000148530 5203_ $$aThe immunomodulatory capacities of mesenchymal stem cells (MSCs) have made them the subject of increased clinical interest for tissue regeneration and repair. We have studied the immunomodulatory capacity of equine MSCs derived from bone marrow (BM-MSCs) and adipose tissue (AT-MSCs) in cocultures with allogeneic peripheral blood mononuclear cells (PBMCs). Different isoforms and concentrations of phytohaemaglutinin (PHA) were tested to determine the best stimulation conditions for PBMC proliferation and a proliferation assay was performed for 7 days to determine the optimal day of stimulation of PBMCs. The effect of the dose and source of MSCs was evaluated in cocultures of 105 PBMCs with different ratios of AT- and BM-MSCs (1:1, 1:10, 1:20 and 1:50). Proliferation rates of the PBMCs were evaluated using BrdU ELISA colorimetric assay. PHA stimulated equine PBMCs reached their peak of growth after 3 days of culture. The immunoassay showed a decrease of the PBMCs growth at high ratio cocultures (1:1 and 1:10). Equine BM-MSCs and AT-MSCs demonstrated an ability to suppress the proliferation of stimulated PBMCs. Although MSCs derived from both sources displayed immunosuppressive effects, AT-MSCs were slightly more potent than BM-MSCs. In addition, the expression of 26 genes coding for different molecules implicated in the immune response was analyzed in cocultures of BM-MSCs and PHA stimulated PBMSCs by reverse transcriptase real time quantitative PCR (RT-qPCR). An upregulation in genes associated with the production of interleukins and cytokines such as TNF-α and TGF-β1 was observed except for IFN-γ whose expression significantly decreased. The variations of interleukins and cytokine receptors showed no clear patterns. COX-1 and COX-2 showed similar expression patterns while INOs expression significantly decreased in the two cell types present in the coculture. Cyclin D2 and IDO-1 showed an increased expression and CD90, ITG-β1 and CD44 expression decreased significantly in BM-MSCs cocultured with PHA stimulated PBMCs. On the contrary, CD6 and VCAM1 expression increased in these cells. With regard to the expression of the five genes involved in antigen presentation, an upregulation was observed in both cocultured MSCs and stimulated PBMCs. This study contributes to the knowledge of the immunoregulatory properties of equine MSCs, which are notably important for the treatment of inflammation processes, such as tendinitis and osteoarthritis.
000148530 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/AGL2011-28609
000148530 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000148530 590__ $$a1.664$$b2015
000148530 591__ $$aVETERINARY SCIENCES$$b26 / 136 = 0.191$$c2015$$dQ1$$eT1
000148530 591__ $$aIMMUNOLOGY$$b128 / 151 = 0.848$$c2015$$dQ4$$eT3
000148530 592__ $$a0.862$$b2015
000148530 593__ $$aVeterinary (miscellaneous)$$c2015$$dQ1
000148530 593__ $$aImmunology$$c2015$$dQ3
000148530 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000148530 700__ $$0(orcid)0000-0001-9818-508X$$aBarrachina, L.$$uUniversidad de Zaragoza
000148530 700__ $$0(orcid)0000-0002-5748-6078$$aÁlvarez-Arguedas, S.$$uUniversidad de Zaragoza
000148530 700__ $$0(orcid)0000-0002-4495-8857$$aRanera, B.
000148530 700__ $$0(orcid)0000-0001-7188-0461$$aRomero, A.$$uUniversidad de Zaragoza
000148530 700__ $$0(orcid)0000-0002-8712-2275$$aVázquez, F.J.$$uUniversidad de Zaragoza
000148530 700__ $$0(orcid)0000-0001-5740-0185$$aZaragoza, P.$$uUniversidad de Zaragoza
000148530 700__ $$aYañez, R.
000148530 700__ $$0(orcid)0000-0001-6016-4726$$aMartín-Burriel, I.$$uUniversidad de Zaragoza
000148530 700__ $$0(orcid)0000-0003-3289-2675$$aRodellar, C.$$uUniversidad de Zaragoza
000148530 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000148530 7102_ $$11008$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Med.Pr.,Sal.Públ.$$cÁrea Microbiología
000148530 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000148530 773__ $$g165, 3-4 (2015), 107-118$$pVet. immunol. immunopathol.$$tVETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY$$x0165-2427
000148530 8564_ $$s745808$$uhttps://zaguan.unizar.es/record/148530/files/texto_completo.pdf$$yPostprint
000148530 8564_ $$s2414000$$uhttps://zaguan.unizar.es/record/148530/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000148530 909CO $$ooai:zaguan.unizar.es:148530$$particulos$$pdriver
000148530 951__ $$a2025-01-21-14:47:35
000148530 980__ $$aARTICLE