000148535 001__ 148535
000148535 005__ 20250121144845.0
000148535 0247_ $$2doi$$a10.1016/j.vetimm.2011.06.033
000148535 0248_ $$2sideral$$a75376
000148535 037__ $$aART-2011-75376
000148535 041__ $$aeng
000148535 100__ $$0(orcid)0000-0002-4495-8857$$aRanera,B.$$uUniversidad de Zaragoza
000148535 245__ $$aImmunophenotype and gene expression profiles of cell surface markers of mesenchymal stem cells derived from equine bone marrow and adipose tissue
000148535 260__ $$c2011
000148535 5060_ $$aAccess copy available to the general public$$fUnrestricted
000148535 5203_ $$aBone marrow and adipose tissue are the two main sources of mesenchymal stem cell (MSC). The aim of this work was to analyse the immunophenotype of 7 surface markers and the expression of a panel of 13 genes coding for cell surface markers in equine bone marrow and adipose tissue-derived MSCs obtained from 9 horses at third passage. The tri-lineage differentiation was confirmed by specific staining. Equine MSCs from both sources were positive for the MSC markers CD29 and CD90, while were negative for CD44, CD73, CD105, CD45 and CD34. The gene expression of these molecules was also evaluated by reverse transcriptase real-time quantitative PCR along with the expression of 5 other MSC markers. Both populations of cells expressed CD13, CD29, CD44, CD49d, CD73, CD90, CD105, CD106, CD146 and CD166 transcripts. Significant differences in gene expression levels between BM- and AT-MSCs were observed for CD44, CD90, CD29 and CD34. Both cell types were negative for CD45 and CD31. The surface antigens tested revealed a similar phenotypic profile between horse and human MSCs, although specific differences in some surface antigens were noticed.
000148535 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000148535 590__ $$a2.076$$b2011
000148535 591__ $$aVETERINARY SCIENCES$$b10 / 141 = 0.071$$c2011$$dQ1$$eT1
000148535 591__ $$aIMMUNOLOGY$$b102 / 138 = 0.739$$c2011$$dQ3$$eT3
000148535 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000148535 700__ $$0(orcid)0000-0003-4796-1872$$aLyahyai,J.
000148535 700__ $$0(orcid)0000-0001-7188-0461$$aRomero,A.$$uUniversidad de Zaragoza
000148535 700__ $$0(orcid)0000-0002-8712-2275$$aVázquez,F. J.$$uUniversidad de Zaragoza
000148535 700__ $$0(orcid)0000-0002-1075-8267$$aRemacha,A. R.$$uUniversidad de Zaragoza
000148535 700__ $$0(orcid)0000-0002-8222-1418$$aBernal,M. L.$$uUniversidad de Zaragoza
000148535 700__ $$0(orcid)0000-0001-5740-0185$$aZaragoza,P.$$uUniversidad de Zaragoza
000148535 700__ $$0(orcid)0000-0003-3289-2675$$aRodellar,C.$$uUniversidad de Zaragoza
000148535 700__ $$0(orcid)0000-0001-6016-4726$$aMartín-Burriel,I.$$uUniversidad de Zaragoza
000148535 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000148535 7102_ $$11005$$2315$$aUniversidad de Zaragoza$$bDpto. Farmacología y Fisiolog.$$cÁrea Farmacología
000148535 7102_ $$11001$$2X$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cProy. investigación DDA
000148535 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000148535 773__ $$g144, 1-2 (2011), 147-154$$pVet. immunol. immunopathol.$$tVETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY$$x0165-2427
000148535 8564_ $$s707753$$uhttps://zaguan.unizar.es/record/148535/files/texto_completo.pdf$$yPostprint
000148535 8564_ $$s838975$$uhttps://zaguan.unizar.es/record/148535/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000148535 909CO $$ooai:zaguan.unizar.es:148535$$particulos$$pdriver
000148535 951__ $$a2025-01-21-14:47:36
000148535 980__ $$aARTICLE