000148536 001__ 148536
000148536 005__ 20250121144845.0
000148536 0247_ $$2doi$$a10.1016/j.tvjl.2012.06.008
000148536 0248_ $$2sideral$$a80637
000148536 037__ $$aART-2013-80637
000148536 041__ $$aeng
000148536 100__ $$0(orcid)0000-0002-4495-8857$$aRanera, B.
000148536 245__ $$aExpansion under hypoxic conditions enhances the chondrogenic potential of equine bone marrow-derived mesenchymal stem cells
000148536 260__ $$c2013
000148536 5060_ $$aAccess copy available to the general public$$fUnrestricted
000148536 5203_ $$aBone marrow-derived mesenchymal stem cells (BM-MSCs) are widely used in regenerative medicine in horses. Most of the molecular characterisations of BM-MSCs have been made at 20% O2, a higher oxygen level than the one surrounding the cells inside the bone marrow. The present work compares the lifespan and the tri-lineage potential of equine BM-MSCs expanded in normoxia (20% O2) and hypoxia (5% O2). No significant differences were found in long-term cultures for osteogenesis and adipogenesis between normoxic and hypoxic expanded BM-MSCs. An up-regulation of the chondrogenesis-related genes (COL2A1, ACAN, LUM, BGL, and COMP) and an increase of the extracellular sulphated glycosaminoglycan content were found in cells that were expanded under hypoxia. These results suggest that the expansion of BM-MSCs in hypoxic conditions enhances chondrogenesis in equine BM-MSCs.
000148536 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000148536 590__ $$a2.165$$b2013
000148536 591__ $$aVETERINARY SCIENCES$$b11 / 131 = 0.084$$c2013$$dQ1$$eT1
000148536 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000148536 700__ $$0(orcid)0000-0002-1075-8267$$aRemacha, A. R.
000148536 700__ $$0(orcid)0000-0002-5748-6078$$aÁlvarez-Arguedas, S.
000148536 700__ $$0(orcid)0000-0001-7453-2470$$aCastiella, T.$$uUniversidad de Zaragoza
000148536 700__ $$0(orcid)0000-0002-8712-2275$$aVázquez, F. J.$$uUniversidad de Zaragoza
000148536 700__ $$0(orcid)0000-0001-7188-0461$$aRomero, A.$$uUniversidad de Zaragoza
000148536 700__ $$0(orcid)0000-0001-5740-0185$$aZaragoza, P.$$uUniversidad de Zaragoza
000148536 700__ $$0(orcid)0000-0001-6016-4726$$aMartin-Burriel, I.$$uUniversidad de Zaragoza
000148536 700__ $$0(orcid)0000-0003-3289-2675$$aRodellar, C.$$uUniversidad de Zaragoza
000148536 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000148536 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000148536 7102_ $$11000$$2020$$aUniversidad de Zaragoza$$bDpto. Anat.Pat.Med.Leg.For.To.$$cArea Anatomía Patológica
000148536 773__ $$g195, 2 (2013), 248-251$$pVet. j.$$tVeterinary Journal$$x1090-0233
000148536 8564_ $$s338349$$uhttps://zaguan.unizar.es/record/148536/files/texto_completo.pdf$$yPostprint
000148536 8564_ $$s2309529$$uhttps://zaguan.unizar.es/record/148536/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000148536 909CO $$ooai:zaguan.unizar.es:148536$$particulos$$pdriver
000148536 951__ $$a2025-01-21-14:47:37
000148536 980__ $$aARTICLE