148536 20250121144845.0 doi 10.1016/j.tvjl.2012.06.008 sideral 80637 ART-2013-80637 eng Ranera, B. (orcid)0000-0002-4495-8857 Expansion under hypoxic conditions enhances the chondrogenic potential of equine bone marrow-derived mesenchymal stem cells 2013 Access copy available to the general public Unrestricted Bone marrow-derived mesenchymal stem cells (BM-MSCs) are widely used in regenerative medicine in horses. Most of the molecular characterisations of BM-MSCs have been made at 20% O2, a higher oxygen level than the one surrounding the cells inside the bone marrow. The present work compares the lifespan and the tri-lineage potential of equine BM-MSCs expanded in normoxia (20% O2) and hypoxia (5% O2). No significant differences were found in long-term cultures for osteogenesis and adipogenesis between normoxic and hypoxic expanded BM-MSCs. An up-regulation of the chondrogenesis-related genes (COL2A1, ACAN, LUM, BGL, and COMP) and an increase of the extracellular sulphated glycosaminoglycan content were found in cells that were expanded under hypoxia. These results suggest that the expansion of BM-MSCs in hypoxic conditions enhances chondrogenesis in equine BM-MSCs. info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 2.165 2013 VETERINARY SCIENCES 11 / 131 = 0.084 2013 Q1 T1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Remacha, A. R. (orcid)0000-0002-1075-8267 Álvarez-Arguedas, S. (orcid)0000-0002-5748-6078 Castiella, T. Universidad de Zaragoza (orcid)0000-0001-7453-2470 Vázquez, F. J. Universidad de Zaragoza (orcid)0000-0002-8712-2275 Romero, A. Universidad de Zaragoza (orcid)0000-0001-7188-0461 Zaragoza, P. Universidad de Zaragoza (orcid)0000-0001-5740-0185 Martin-Burriel, I. Universidad de Zaragoza (orcid)0000-0001-6016-4726 Rodellar, C. Universidad de Zaragoza (orcid)0000-0003-3289-2675 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 1000 020 Universidad de Zaragoza Dpto. Anat.Pat.Med.Leg.For.To. Area Anatomía Patológica 195, 2 (2013), 248-251 Vet. j. Veterinary Journal 1090-0233 338349 http://zaguan.unizar.es/record/148536/files/texto_completo.pdf Postprint 2309529 http://zaguan.unizar.es/record/148536/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:148536 articulos driver 2025-01-21-14:47:37 ARTICLE