000148539 001__ 148539
000148539 005__ 20250125205839.0
000148539 0247_ $$2doi$$a10.1016/j.joca.2017.06.007
000148539 0248_ $$2sideral$$a99596
000148539 037__ $$aART-2017-99596
000148539 041__ $$aeng
000148539 100__ $$0(orcid)0000-0002-8731-4269$$aJaime, P.$$uUniversidad de Zaragoza
000148539 245__ $$aCD56+/CD16- Natural Killer cells expressing the inflammatory protease granzyme A are enriched in synovial fluid from patients with osteoarthritis
000148539 260__ $$c2017
000148539 5060_ $$aAccess copy available to the general public$$fUnrestricted
000148539 5203_ $$aObjective: Natural killer (NK) cells have been involved in the pathology of different inflammatory and autoimmune disorders. Inflammation is an important regulator of osteoarthritis (OA), but the molecular and cellular mechanisms regulating this process are not well defined. Design To understand the role of NK cells in OA, we have compared the phenotype (CD56 subsets and perforin and granzyme expression) and cytotoxic function of NK cells in peripheral blood and synovial fluid from patients with OA undergoing total knee arthroplasty.
Results: In contrast to peripheral blood lymphocytes (PBLs), the majority of NK cells from the synovial fluid were CD56brightCD16(-) cells. As expected the expression of the cytolytic mediators perforin and granzyme B in CD56brightCD16(-) cells was low and correlated with a poor cytotoxic potential against K562 sensitive target cells. Surprisingly, this low cytotoxic NK cell subset expressed high levels of granzyme A (a protease recently characterized as a key modulator of inflammation in mouse models) in synovial fluid but not in peripheral blood. The presence of the CD56(+)brightCD16(-) cells expressing granzyme A correlated with increased levels of pro-inflammatory cytokines in synovial fluid from OA patients.
Conclusion: Our results indicate that NK cells from the synovium of patients with OA, which present an immunoregulatory non-cytotoxic phenotype, show different phenotype comparing with NK cells from peripheral blood, especially expressing granzyme A, a pro-inflammatory molecule which may contribute to the establishment of chronic articular inflammation in this type of patients.
000148539 536__ $$9info:eu-repo/grantAgreement/ES/DGA-FSE/B27$$9info:eu-repo/grantAgreement/ES/MINECO-FEDER/SAF2011-25390$$9info:eu-repo/grantAgreement/ES/MINECO/SAF2014-54763-C2-1-R$$9info:eu-repo/grantAgreement/EUR/TRAIN2/SOE2-P1-E-341
000148539 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000148539 590__ $$a5.454$$b2017
000148539 591__ $$aRHEUMATOLOGY$$b4 / 30 = 0.133$$c2017$$dQ1$$eT1
000148539 591__ $$aORTHOPEDICS$$b2 / 77 = 0.026$$c2017$$dQ1$$eT1
000148539 592__ $$a2.497$$b2017
000148539 593__ $$aBiomedical Engineering$$c2017$$dQ1
000148539 593__ $$aRheumatology$$c2017$$dQ1
000148539 593__ $$aOrthopedics and Sports Medicine$$c2017$$dQ1
000148539 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000148539 700__ $$aGarcía-Guerrero, N.$$uUniversidad de Zaragoza
000148539 700__ $$aEstella, R.
000148539 700__ $$0(orcid)0000-0003-0154-0730$$aPardo, J.$$uUniversidad de Zaragoza
000148539 700__ $$0(orcid)0000-0003-2587-7506$$aGarcía-Álvarez, F.$$uUniversidad de Zaragoza
000148539 700__ $$0(orcid)0000-0003-3043-147X$$aMartínez-Lostao, L.$$uUniversidad de Zaragoza
000148539 7102_ $$11008$$2566$$aUniversidad de Zaragoza$$bDpto. Microb.Med.Pr.,Sal.Públ.$$cÁrea Inmunología
000148539 7102_ $$11004$$2830$$aUniversidad de Zaragoza$$bDpto. Cirugía,Ginecol.Obstetr.$$cÁrea Traumatología y Ortopedia
000148539 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000148539 773__ $$g25, 10 (2017), 1708-1718$$pOsteoarthr. cartil.$$tOsteoarthritis and cartilage$$x1063-4584
000148539 8564_ $$s559592$$uhttps://zaguan.unizar.es/record/148539/files/texto_completo.pdf$$yPostprint
000148539 8564_ $$s1471966$$uhttps://zaguan.unizar.es/record/148539/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000148539 909CO $$ooai:zaguan.unizar.es:148539$$particulos$$pdriver
000148539 951__ $$a2025-01-25-20:57:57
000148539 980__ $$aARTICLE