000148542 001__ 148542 000148542 005__ 20250120165541.0 000148542 0247_ $$2doi$$a10.1111/are.13668 000148542 0248_ $$2sideral$$a106429 000148542 037__ $$aART-2018-106429 000148542 041__ $$aeng 000148542 100__ $$0(orcid)0000-0001-9907-2941$$aMitjana, O.$$uUniversidad de Zaragoza 000148542 245__ $$aSimultaneous effect of sex and dose on efficacy of clove oil, tricaine methanesulfonate, 2-phenoxyethanol and propofol as anaesthetics in guppies, Poecilia reticulata (Peters) 000148542 260__ $$c2018 000148542 5060_ $$aAccess copy available to the general public$$fUnrestricted 000148542 5203_ $$aWe studied the simultaneous effect of sex and dose on anaesthesia efficacy to estimate, if possible, the lowest effective dose (LED) for clove oil, tricaine methanesulphonate (MS-222), 2-phenoxyethanol (2-PE) and propofol in mature guppies. LED is the lowest dose needed to reach A5 stage in a mean time of 3 min, with mean recovery (R5) time of 5 min. We used four doses/anaesthetic: 25, 50, 75 and 100 mg/L for clove oil; 120, 140, 160 and 180 mg/L for MS-222; 800, 1, 000, 1, 200 and 1, 400 mg/L for 2-PE, and 7.50, 8.25, 10.00 and 11.25 mg/L for propofol. Each dose was tested on 10 females and 10 males. Morbidity, mortality and behavioural changes were checked on two control groups (10 males and 10 females/group). Sedation (A3), A5 and R5 times were recorded. Significant interactive effect dose*sex on A5 time was found for each anaesthetic agent (pdose*sex<.05). Except for MS-222 (pdose*sex =.284), significant interactive effect dose * sex on R5 time was found (pdose*sex <.05). A5 time in females tended to be greater than in males, but, in general, R5 times were longer in males. Body size differences between males and females could explain these differences in MS-222 on A5 time and for clove oil, 2-PE and propofol on R5 time. No dose simultaneous meet LED's conditions relating to both A5 and R5 times; therefore the lowest doses inducing A5 in a mean time of 3 min could be a safe guideline for anaesthetic procedure in both male and females. 000148542 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000148542 590__ $$a1.502$$b2018 000148542 591__ $$aFISHERIES$$b30 / 52 = 0.577$$c2018$$dQ3$$eT2 000148542 592__ $$a0.646$$b2018 000148542 593__ $$aAquatic Science$$c2018$$dQ2 000148542 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000148542 700__ $$0(orcid)0000-0001-6178-9891$$aBonastre, C.$$uUniversidad de Zaragoza 000148542 700__ $$0(orcid)0000-0001-5026-5144$$aTejedor, M.T.$$uUniversidad de Zaragoza 000148542 700__ $$0(orcid)0000-0001-7106-0068$$aGarza, L. 000148542 700__ $$aEsteban, J. 000148542 700__ $$0(orcid)0000-0001-6964-7011$$aFalceto, M.V.$$uUniversidad de Zaragoza 000148542 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética 000148542 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal 000148542 773__ $$g49, 6 (2018), 2140-2146$$pAquac. res.$$tAquaculture Research$$x1355-557X 000148542 8564_ $$s159438$$uhttps://zaguan.unizar.es/record/148542/files/texto_completo.pdf$$yVersión publicada 000148542 8564_ $$s1997644$$uhttps://zaguan.unizar.es/record/148542/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000148542 909CO $$ooai:zaguan.unizar.es:148542$$particulos$$pdriver 000148542 951__ $$a2025-01-20-14:52:43 000148542 980__ $$aARTICLE