148544 20250121144846.0 doi 10.1002/jbm.b.34009 sideral 104431 ART-2018-104431 eng Gimeno, María Antibiotic-eluting orthopedic device to prevent early implant associated infections: Efficacy, biocompatibility and biodistribution studies in an ovine model 2018 Access copy available to the general public Unrestricted Infection of orthopedic devices is a major complication in the postsurgical period generating important health issues and economic consequences. Prevention strategies could be based on local release of antibiotics from the orthopedic device itself to avoid adhesion and growth of bacteria. The purpose of this work is to demonstrate the efficiency to prevent these infections by a cefazolin-eluting, perforated stainless steel implant in an in vivo ovine model. The device was placed in the tibia of sheep, one group receiving cefazolin-loaded implants whereas the control group received empty implants. All implants were experimentally infected by direct inoculation of Staphylococcus aureus ATCC 6538. In vitro cytotoxicological studies were also performed to check the effect of antibiotic on cell viability, integrity, and cycle. Results showed that sheep receiving cefazolin-loaded devices were able to avoid implant-associated infections, with normal tissue healing process. The antibiotic release followed a local concentric pattern as demonstrated by high-performance liquid chromatography detection in tissues. The in vitro results indicate the lack of relevant cytotoxic effects for the maximum antibiotic concentration released by the device. These results demonstrate the efficiency and safety of cefazolin-eluting implants in an ovine model to prevent early postsurgical infections of orthopedic devices info:eu-repo/grantAgreement/EC/FP7/614715/EU/A Photo-triggered On-demand Drug Delivery System for Chronic Pain/NANOHEDONISM info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 2.674 2018 ENGINEERING, BIOMEDICAL 29 / 80 = 0.363 2018 Q2 T2 MATERIALS SCIENCE, BIOMATERIALS 19 / 32 = 0.594 2018 Q3 T2 0.658 2018 Biomedical Engineering 2018 Q2 Biomaterials 2018 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Pinczowski, Pedro (orcid)0000-0002-9210-6026 Mendoza, Gracia (orcid)0000-0003-2293-363X Asín, Javier Universidad de Zaragoza (orcid)0000-0002-6178-4801 Vázquez, Francisco J. Universidad de Zaragoza (orcid)0000-0002-8712-2275 Vispe, Eugenio (orcid)0000-0002-4921-4734 García-Álvarez, Felícito Universidad de Zaragoza (orcid)0000-0003-2587-7506 Pérez, Marta Universidad de Zaragoza (orcid)0000-0002-8133-2124 Santamaría, Jesús Universidad de Zaragoza (orcid)0000-0002-8701-9745 Arruebo, Manuel Universidad de Zaragoza (orcid)0000-0003-3165-0156 Luján, Lluís Universidad de Zaragoza (orcid)0000-0002-2053-9842 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1001 025 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Anatom.Anatom.Patológ.Com 1004 830 Universidad de Zaragoza Dpto. Cirugía,Ginecol.Obstetr. Área Traumatología y Ortopedia 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 106 , 5 (2018), 1976-1986 J. biomed. mater. res., Part B Appl. biomater. Journal of Biomedical Materials Research - Part B Applied Biomaterials 1552-4973 877103 http://zaguan.unizar.es/record/148544/files/texto_completo.pdf Postprint 1414480 http://zaguan.unizar.es/record/148544/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:148544 articulos driver 2025-01-21-14:47:39 ARTICLE