148553 20250127144949.0 doi 10.1016/j.meegid.2020.104184 sideral 116832 ART-2020-116832 eng Comin, J. Investigation of a rapidly spreading tuberculosis outbreak using whole-genome sequencing 2020 Access copy available to the general public Unrestricted This paper describes the application of whole-genome sequencing (WGS) to investigate an outbreak of Mycobacterium tuberculosis occurring in Aragon, Spain, where strains have been submitted to genotyping since 2004. The responsible outbreak strain appeared in our region first in 2014 and it spread to 14 patients in the following three years. WGS found low variability between the isolates with none of the SNPs differences detected more than once, all of which were attributed to a recent transmission. Although two ambiguous bases linked two cases with those who presented the SNP in the same position, the establishment of a definitive transmission route was not possible. The epidemiological data supported the existence of a super-spreader, probably responsible for the majority of the cases involved since there was a two-year delay in diagnoses among cases. This fact would also help explaining the low variability found. The index case was not identified, possibly because it was not diagnosed in Aragon. In addition WGS characterised the strain as a Linage 4.3.3/LAM family and corroborated the susceptibility to anti-tuberculosis drugs observed by the clinical laboratories. This work shows the need to have epidemiological data to support the genomic data in order to clarify the evolution of tuberculosis outbreaks. info:eu-repo/grantAgreement/ES/DGA-FEDER/Construyendo Europa desde Aragón info:eu-repo/grantAgreement/ES/ISCIII/FIS15-0317 info:eu-repo/grantAgreement/ES/ISCIII/FIS18-0336 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 3.342 2020 INFECTIOUS DISEASES 51 / 92 = 0.554 2020 Q3 T2 1.085 2020 Ecology, Evolution, Behavior and Systematics 2020 Q1 Genetics 2020 Q1 Molecular Biology 2020 Q1 Microbiology 2020 Q1 Microbiology (medical) 2020 Q1 Infectious Diseases 2020 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Chaure, A. Cebollada, A. (orcid)0000-0002-3312-7759 Ibarz, D. Viñuelas, J. Universidad de Zaragoza (orcid)0000-0003-2044-6782 Vitoria, M. A. (orcid)0000-0003-4198-6622 Iglesias, M. J. Universidad de Zaragoza (orcid)0000-0003-2266-8602 Samper, S. Universidad de Zaragoza (orcid)0000-0002-3011-2519 1011 630 Universidad de Zaragoza Dpto. Microb.Ped.Radio.Sal.Pú. Área Microbiología 1011 615 Universidad de Zaragoza Dpto. Microb.Ped.Radio.Sal.Pú. Área Medic.Prevent.Salud Públ. 81 (2020), 104184 [7 pp.] INFECTION GENETICS AND EVOLUTION INFECTION GENETICS AND EVOLUTION 1567-1348 701857 http://zaguan.unizar.es/record/148553/files/texto_completo.pdf Versión publicada 2385682 http://zaguan.unizar.es/record/148553/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:148553 articulos driver 2025-01-27-14:45:29 ARTICLE