148566 20250120165542.0 doi 10.1016/j.foodchem.2021.131016 sideral 128360 ART-2022-128360 eng Aymard, Chloé Development of a new dual electrochemical immunosensor for a rapid and sensitive detection of enrofloxacin in meat samples 2022 A novel dual electrochemical immunosensor was fabricated for the rapid and sensitive detection of enrofloxacin (EF) antibiotic in meat. Anti-quinolone antibody was immobilized onto screen-printed dual carbon electrodes via carbodiimide coupling. A new electrochemical probe was synthesized by conjugating difloxacin and aminoferrocene, whose oxidation was measured at + 0.2 V vs. Ag/AgCl by differential pulse voltammetry. The detection principle was based on the competitive binding of this conjugate and free EF on immobilized antibodies. The proposed immunosensor allowed detection of EF at concentrations ranging from 0.005 mu g.mL(-1) to 0.01 mu g.mL(-1) with a detection limit of 0.003 mu g.mL(-1). The immunosensor was stable for at least 1 month at 4 degrees C and displayed a good specificity for other fluoroquinolones. The new dual electrode design offered an improved accuracy as one electrode was used as negative control. The efficiency of the sensor and the adequacy of the extraction process were finally validated by detecting EF in different meat samples. info:eu-repo/semantics/closedAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 8.8 2022 CHEMISTRY, APPLIED 5 / 72 = 0.069 2022 Q1 T1 NUTRITION & DIETETICS 5 / 87 = 0.057 2022 Q1 T1 FOOD SCIENCE & TECHNOLOGY 9 / 142 = 0.063 2022 Q1 T1 1.624 2022 Analytical Chemistry 2022 Q1 Medicine (miscellaneous) 2022 Q1 Food Science 2022 Q1 14.9 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Kanso, Hussein Serrano, María Jesús (orcid)0000-0003-3850-9833 Pagán, Rafael Universidad de Zaragoza (orcid)0000-0002-0238-6328 Noguer, Thierry Istamboulie, Georges. 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 370 (2022), 131016 [8 pp] Food chem. Food Chemistry 0308-8146 1900696 http://zaguan.unizar.es/record/148566/files/texto_completo.pdf Versión publicada 2591088 http://zaguan.unizar.es/record/148566/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:148566 articulos driver 2025-01-20-14:53:15 ARTICLE