148591 20250120165542.0 doi 10.1080/10717544.2018.1474966 sideral 135596 ART-2018-135596 eng Ciriza, Jesús (orcid)0000-0002-8666-622X Graphene oxide enhances alginate encapsulated cells viability and functionality while not affecting the foreign body response 2018 The combination of protein-coated graphene oxide (GO) and microencapsulation technology has moved a step forward in the challenge of improving long-term alginate encapsulated cell survival and sustainable therapeutic protein release, bringing closer its translation from bench to the clinic. Although this new approach in cell microencapsulation represents a great promise for long-term drug delivery, previous studies have been performed only with encapsulated murine C2C12 myoblasts genetically engineered to secrete murine erythropoietin (C2C12-EPO) within 160 µm diameter hybrid alginate protein-coated GO microcapsules implanted into syngeneic mice. Here, we show that encapsulated C2C12-EPO myoblasts survive longer and release more therapeutic protein by doubling the micron diameter of hybrid alginate-protein-coated GO microcapsules to 380 µm range. Encapsulated mesenchymal stem cells (MSC) genetically modified to secrete erythropoietin (D1-MSCs-EPO) within 380 µm-diameter hybrid alginate-protein-coated GO microcapsules confirmed this improvement in survival and sustained protein release in vitro. This improved behavior is reflected in the hematocrit increase of allogeneic mice implanted with both encapsulated cell types within 380 µm diameter hybrid alginate-protein-coated GO microcapsules, showing lower immune response with encapsulated MSCs. These results provide a new relevant step for the future clinical application of protein-coated GO on cell microencapsulation. Access copy available to the general public Unrestricted info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 3.829 2018 PHARMACOLOGY & PHARMACY 60 / 266 = 0.226 2018 Q1 T1 0.937 2018 Pharmaceutical Science 2018 Q1 Medicine (miscellaneous) 2018 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Sáenz del Burgo, Laura Gurruchaga, Haritz Borras, Francesc E. Franquesa, Marcella Orive, Gorka Hernández, Rosa María Pedraz, José Luis 25, 1 (2018), 1147-1160 Drug deliv. Drug Delivery 1071-7544 2571824 http://zaguan.unizar.es/record/148591/files/texto_completo.pdf Versión publicada 3105658 http://zaguan.unizar.es/record/148591/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:148591 articulos driver 2025-01-20-14:53:45 ARTICLE