000148617 001__ 148617
000148617 005__ 20250120165542.0
000148617 0247_ $$2doi$$a10.1016/j.arbres.2024.12.005
000148617 0248_ $$2sideral$$a141351
000148617 037__ $$aART-2024-141351
000148617 041__ $$aeng
000148617 100__ $$0(orcid)0000-0002-5228-248X$$aSanz-Rubio, David
000148617 245__ $$aCirculating Exosomal MicroRNAs and Subclinical Atherosclerosis in Obstructive Sleep Apnea
000148617 260__ $$c2024
000148617 5060_ $$aAccess copy available to the general public$$fUnrestricted
000148617 5203_ $$aBackground. Obstructive sleep apnea (OSA) has been associated with thepathogenesis of atherogenesis. Exosomes and their microRNA (miRNA) cargocould play a major role in this process.Objectives. To determine plasma exosomal miRNA and their relationship withatherosclerosis in patients with OSA.Methods. From the EPIOSA cohort, we selected 50 OSA patients (apnea-hypopnea index–AHI ≥ 10) and 16 age- and sex-matched healthy subjects (AHI≤ 10) for the derivation analysis. The validation assay was composed by 88 OSApatients and 24 matched controls. OSA patients were categorized according toprevalent subclinical atherosclerosis (SA) as defined by ≥ 1 plaque in carotidultrasound. All participants were free of prevalent chronic comorbid conditions.Plasma-derived exosomes were isolated by precipitation, and miRNAs wereevaluated by real-time quantitative PCR.Results. SA was present in 34% of OSA patients. Three exosomal miRNAs wereoverexpressed in patients with OSA and SA at the derivation study: miR-21(relative expression, RE = 2.91), miR-145 (RE = 2.12) and miR-320a (RE =4.06). ROC curve analysis showed a high predictive value for SA of miR-320a(area under curve AUC = 0.813). In the validation cohort, miR-320a alsoexhibited an overexpression of RE = 2.66 among patients with OSA and SA, withan AUC of 0.798. Together with AHI and APO B, miR-320a, was independentlyassociated with SA. In addition, miR-320a correlate with the progression of intimamedia thickness from baseline to after 1 year of follow-up. Finally, miR-320a wasnot reduced after 1 year of follow-up independiently of the treatment with CPAP.3Conclusions. In patients with OSA, exosomal miR-320a is associated to thepresence of subclinical atherosclerosis and could be a useful marker ofcardiovascular risk in these patients
000148617 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B22-20R$$9info:eu-repo/grantAgreement/ES/DGA-CUS/1466-2020$$9info:eu-repo/grantAgreement/ES/ISCIII CD22-00033$$9info:eu-repo/grantAgreement/ES/ISCIII/PI15-01441$$9info:eu-repo/grantAgreement/ES/ISCIII/PI18-01524$$9info:eu-repo/grantAgreement/ES/ISCIII/PI21-01954$$9info:eu-repo/grantAgreement/ES/MICINN/RYC2019--027831-I
000148617 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000148617 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000148617 700__ $$0(orcid)0000-0001-6016-4726$$aMartín-Burriel, Inmaculada$$uUniversidad de Zaragoza
000148617 700__ $$0(orcid)0000-0001-6351-0444$$aRodríguez, Jorge$$uUniversidad de Zaragoza
000148617 700__ $$aMarín-Oto, Marta$$uUniversidad de Zaragoza
000148617 700__ $$aKhalyfa, Abdelnaby
000148617 700__ $$aSánchez-de-la-Torre, Manuel
000148617 700__ $$aGozal, David
000148617 700__ $$0(orcid)0000-0001-9096-2294$$aMarin, Jose M.$$uUniversidad de Zaragoza
000148617 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000148617 7102_ $$11007$$2610$$aUniversidad de Zaragoza$$bDpto. Medicina, Psiqu. y Derm.$$cArea Medicina
000148617 773__ $$pArch. bronconeumol.$$tArchivos de Bronconeumologia$$x0300-2896
000148617 8564_ $$s638968$$uhttps://zaguan.unizar.es/record/148617/files/texto_completo.pdf$$yPostprint$$zinfo:eu-repo/date/embargoEnd/2026-01-09
000148617 8564_ $$s1205652$$uhttps://zaguan.unizar.es/record/148617/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint$$zinfo:eu-repo/date/embargoEnd/2026-01-09
000148617 909CO $$ooai:zaguan.unizar.es:148617$$particulos$$pdriver
000148617 951__ $$a2025-01-20-14:54:13
000148617 980__ $$aARTICLE