000148627 001__ 148627 000148627 005__ 20250120165542.0 000148627 0247_ $$2doi$$a10.1016/j.rvsc.2022.02.009 000148627 0248_ $$2sideral$$a141782 000148627 037__ $$aART-2022-141782 000148627 041__ $$aeng 000148627 100__ $$0(orcid)0000-0001-7106-0068$$aGarza-Moreno, Laura$$uUniversidad de Zaragoza 000148627 245__ $$aEnvironmental detection of Mycoplasma hyopneumoniae in breed-to-wean farms 000148627 260__ $$c2022 000148627 5060_ $$aAccess copy available to the general public$$fUnrestricted 000148627 5203_ $$aThere is a need to develop cost-effective and non-invasive approaches to sample large populations to evaluate the disease status of breeding herds. In this study we assessed the detection of the M. hyopneumoniae genetic material in environmental surfaces and air of farrowing rooms, and skin (udder, snout and vagina) of lactating sows at weaning, in farms having different M. hyopneumoniae infection status (negative, positive sub-clinically infected and positive clinically affected). Mycoplasma hyopneumoniae was detected in air, air deposition particles, dam and stall surfaces of the positive clinically affected herd. Mycoplasma hyopneumoniae could only be detected in dam and stall surfaces in sub-clinically infected herds. Mycoplasma hyopneumoniae was not detected in all samples collected in the negative herd. The cycle threshold of the positive PCR samples were not statistically different between sample types or farms. However, a significant difference (p < 0.05) was observed in the percentage of positive samples between the positive clinically affected farm and the rest. Likewise, M. hyopneumoniae was detected in the environment and surfaces at weaning in positive breeding herds. Further testing and validation is recommended for environmental and surface samples before they can be employed as part of the M. hyopneumoniae diagnostic process. In addition, results from this study highlight potential sources of M. hyopneumoniae infection for piglets in breeding herds, especially during an outbreak. 000148627 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/ 000148627 590__ $$a2.4$$b2022 000148627 591__ $$aVETERINARY SCIENCES$$b29 / 144 = 0.201$$c2022$$dQ1$$eT1 000148627 592__ $$a0.577$$b2022 000148627 593__ $$aVeterinary (miscellaneous)$$c2022$$dQ1 000148627 594__ $$a4.1$$b2022 000148627 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000148627 700__ $$aVilalta, Carles 000148627 700__ $$aPieters, Maria 000148627 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal 000148627 773__ $$g145 (2022), 188-192$$pRes. Vet. Sci.$$tRESEARCH IN VETERINARY SCIENCE$$x0034-5288 000148627 8564_ $$s751648$$uhttps://zaguan.unizar.es/record/148627/files/texto_completo.pdf$$yVersión publicada 000148627 8564_ $$s2452754$$uhttps://zaguan.unizar.es/record/148627/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000148627 909CO $$ooai:zaguan.unizar.es:148627$$particulos$$pdriver 000148627 951__ $$a2025-01-20-14:54:23 000148627 980__ $$aARTICLE