148654 20260217205547.0 doi 10.3168/jds.2024-25046 sideral 141946 ART-2024-141946 eng Belanche, Alejandro Universidad de Zaragoza (orcid)0000-0001-5880-6021 Feed additives for methane mitigation: A guideline to uncover the mode of action of antimethanogenic feed additives for ruminants 2024 Access copy available to the general public Unrestricted This publication aims to provide guidelines of the knowledge required and the potential research to be con-ducted in order to understand the mode of action of anti-methanogenic feed additives (AMFA). In the first part of the paper, we classify AMFA into 4 categories according to their mode of action: (1) lowering dihydrogen (H2) production; (2) inhibiting methanogens; (3) promoting alternative H2-incorporating pathways; and (4) oxidizing methane (CH4). The second part of the paper presents questions that guide the research to identify the mode of action of an AMFA on the rumen CH4 production from 5 different perspectives: (1) microbiology; (2) cell and molecular biochemistry; (3) microbial ecology; (4) animal metabolism; and (5) cross-cutting aspects. Rec-ommendations are provided to address various research questions within each perspective, along with examples of how aspects of the mode of action of AMFA have been elucidated before. In summary, this paper offers timely and comprehensive guidelines to better understand and reveal the mode of action of current and emerging AMFA. info:eu-repo/grantAgreement/ES/AEI/PID2021-12306OB-I00 info:eu-repo/grantAgreement/ES/MICINN/RYC2019-027764-I/AEI/10.13039/501100011033 info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es 4.4 2024 AGRICULTURE, DAIRY & ANIMAL SCIENCE 4 / 86 = 0.047 2024 Q1 T1 FOOD SCIENCE & TECHNOLOGY 51 / 182 = 0.28 2024 Q2 T1 1.25 2024 Animal Science and Zoology 2024 Q1 Genetics 2024 Q1 Food Science 2024 Q1 7.8 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Bannink, André Dijkstra, Jan Durmic, Zoey Garcia, Florencia Santos, Fernanda G. Huws, Sharon Jeyanathan, Jeyamalar Lund, Peter Mackie, Roderick I. McAllister, Tim A. Morgavi, Diego P. Muetzel, Stefan Pitta, Dipti W. Yáñez-Ruiz, David R. Ungerfeld, Emilio M. 2008 700 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Producción Animal 108, 1 (2024), 375-394 J. dairy sci. Journal of Dairy Science 0022-0302 1147744 http://zaguan.unizar.es/record/148654/files/texto_completo.pdf Versión publicada 3396658 http://zaguan.unizar.es/record/148654/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:148654 articulos driver 2026-02-17-20:38:52 ARTICLE