148684 20250121150753.0 doi 10.1016/j.ijfoodmicro.2017.06.002 sideral 100757 ART-2017-100757 eng Chueca, Beatriz Global transcriptional response of Escherichia coli MG1655 cells exposed to the oxygenated monoterpenes citral and carvacrol 2017 DNA microarrays were used to study the mechanism of bacterial inactivation by carvacrol and citral. After 10-min treatments of Escherichia coli MG1655 cells with 100 and 50 ppm of carvacrol and citral, 76 and 156 genes demonstrated significant transcriptional differences (p = 0.05), respectively. Among the up-regulated genes after carvacrol treatment, we found gene coding for multidrug efflux pumps (acrA, mdtM), genes related to phage shock response (pspA, pspB, pspC, pspD, pspF and pspG), biosynthesis of arginine (argC, argG, artJ), and purine nucleotides (purC, purM). In citral-treated cells, transcription of purH and pyrB and pyrI was 2 times higher. Deletion of several differentially expressed genes confirmed the role of ygaV, yjbO, pspC, sdhA, yejG and ygaV in the mechanisms of E. coli inactivation by carvacrol and citral. These results would indicate that citral and carvacrol treatments cause membrane damage and activate metabolism through the production of nucleotides required for DNA and RNA synthesis and metabolic processes. Comparative transcriptomics of the response of E. coli to a heat treatment, which caused a significant change of the transcription of 1422 genes, revealed a much weaker response to both individual constituents of essential oils (ICs).·Thus, inactivation by citral or carvacrol was not multitarget in nature. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/ES/MINECO/AGL2012-32165 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 3.451 2017 FOOD SCIENCE & TECHNOLOGY 17 / 133 = 0.128 2017 Q1 T1 MICROBIOLOGY 43 / 125 = 0.344 2017 Q2 T2 1.366 2017 Food Science 2017 Q1 Safety, Risk, Reliability and Quality 2017 Q1 Medicine (miscellaneous) 2017 Q1 Microbiology 2017 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Pérez-Sáez, Elisa Pagán, Rafael Universidad de Zaragoza (orcid)0000-0002-0238-6328 García-Gonzalo, Diego Universidad de Zaragoza (orcid)0000-0002-7629-8101 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 257 (2017), 49-57 Int. j. food microbiol. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY 0168-1605 1054923 http://zaguan.unizar.es/record/148684/files/texto_completo.pdf Postprint 659501 http://zaguan.unizar.es/record/148684/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:148684 articulos driver 2025-01-21-14:43:23 ARTICLE