000148696 001__ 148696
000148696 005__ 20250121150753.0
000148696 0247_ $$2doi$$a10.1016/j.ijfoodmicro.2020.108837
000148696 0248_ $$2sideral$$a119840
000148696 037__ $$aART-2020-119840
000148696 041__ $$aeng
000148696 100__ $$ade Souza Pedrosa, G.T.
000148696 245__ $$aPhysiological alterations involved in inactivation of autochthonous spoilage bacteria in orange juice caused by Citrus essential oils and mild heat
000148696 260__ $$c2020
000148696 5203_ $$aThis study investigated physiological alterations involved in the inactivation of Levilactobacillus (L.) brevis and Leuconostoc (Lc.) mesenteroides in orange juice caused by Citrus lemon essential oil (CLEO) and C. reticulata essential oil (CREO) alone and combined with mild heat treatment (MHT). Damage in DNA, membrane integrity, membrane potential, metabolic and efflux activity of bacterial cells were measured after exposure (6 and 12 min) to CLEO or CREO (0.5 µL/mL) and/or MHT (54 °C) using flow cytometry. Limonene was the major constituent in CLEO (66.4%) and CREO (89.4%). The size of the damaged cell subpopulations increased (p < 0.05) after longer exposure time and varied with the tested essential oil and/or bacterial isolate. After exposure to CLEO and CREO alone, the cell subpopulations with damage in measured physiological functions were in a range of 19.6–66.8% and 23.8–75.9%, respectively. Exposure to CREO resulted in larger Lc. mesenteroides cell subpopulations (35.4–68.7%) with damaged DNA, permeabilized and depolarized membrane and compromised metabolic or efflux activity compared to L. brevis (23.8–58.0%). In contrast, exposure to CLEO led to higher damaged L. brevis cell subpopulations (35.1–77%) compared to Lc. mesenteroides (25.3–36.6%). Exposure to combined treatments (CLEO or CREO and MHT) affected the measured physiological functions in almost the entire L. brevis and Lc. mesenteroides cell population (up to 99%), although the damage extension on each isolate varied with tested essential oil. Results show that inactivation of L. brevis and Lc. mesenteroides cells caused by CLEO and CREO alone and combined with MHT in orange juice involves a multi-target action, which causes DNA damage, altered permeability and depolarization of membrane and compromised metabolic and efflux activities.
000148696 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/AGL2015-69565-P
000148696 540__ $$9info:eu-repo/semantics/closedAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000148696 590__ $$a5.277$$b2020
000148696 591__ $$aFOOD SCIENCE & TECHNOLOGY$$b25 / 143 = 0.175$$c2020$$dQ1$$eT1
000148696 591__ $$aMICROBIOLOGY$$b36 / 135 = 0.267$$c2020$$dQ2$$eT1
000148696 592__ $$a1.31$$b2020
000148696 593__ $$aFood Science$$c2020$$dQ1
000148696 593__ $$aSafety, Risk, Reliability and Quality$$c2020$$dQ1
000148696 593__ $$aMicrobiology$$c2020$$dQ1
000148696 593__ $$aMedicine (miscellaneous)$$c2020$$dQ1
000148696 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000148696 700__ $$ade Souza, E.L.
000148696 700__ $$ade Melo, A.N.F.
000148696 700__ $$ada Cruz Almeida, E.T.
000148696 700__ $$ade Sousa Guedes, J.P.
000148696 700__ $$ade Carvalho, R.J.
000148696 700__ $$0(orcid)0000-0002-0238-6328$$aPagán, R.$$uUniversidad de Zaragoza
000148696 700__ $$aMagnani, M.
000148696 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000148696 773__ $$g334 (2020), 108837 [8 pp]$$pInt. j. food microbiol.$$tINTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY$$x0168-1605
000148696 8564_ $$s653460$$uhttps://zaguan.unizar.es/record/148696/files/texto_completo.pdf$$yVersión publicada
000148696 8564_ $$s2612304$$uhttps://zaguan.unizar.es/record/148696/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000148696 909CO $$ooai:zaguan.unizar.es:148696$$particulos$$pdriver
000148696 951__ $$a2025-01-21-14:43:37
000148696 980__ $$aARTICLE