| Página principal > Artículos > Effect of hypoxia on equine mesenchymal stem cells derived from bone marrow and adipose tissue > MARC |
000148715 001__ 148715 000148715 005__ 20250121150753.0 000148715 0247_ $$2doi$$a10.1186/1746-6148-8-142 000148715 0248_ $$2sideral$$a80193 000148715 037__ $$aART-2012-80193 000148715 041__ $$aeng 000148715 100__ $$0(orcid)0000-0002-4495-8857$$aRanera, B.$$uUniversidad de Zaragoza 000148715 245__ $$aEffect of hypoxia on equine mesenchymal stem cells derived from bone marrow and adipose tissue 000148715 260__ $$c2012 000148715 5060_ $$aAccess copy available to the general public$$fUnrestricted 000148715 5203_ $$aBackground Mesenchymal stem cells (MSCs) derived from bone marrow (BM-MSCs) and adipose tissue (AT-MSCs) are being applied to equine cell therapy. The physiological environment in which MSCs reside is hypoxic and does not resemble the oxygen level typically used in in vitro culture (20% O2). This work compares the growth kinetics, viability, cell cycle, phenotype and expression of pluripotency markers in both equine BM-MSCs and AT-MSCs at 5% and 20% O2. Results At the conclusion of culture, fewer BM-MSCs were obtained in hypoxia than in normoxia as a result of significantly reduced cell division. Hypoxic AT-MSCs proliferated less than normoxic AT-MSCs because of a significantly higher presence of non-viable cells during culture. Flow cytometry analysis revealed that the immunophenotype of both MSCs was maintained in both oxygen conditions. Gene expression analysis using RT-qPCR showed that statistically significant differences were only found for CD49d in BM-MSCs and CD44 in AT-MSCs. Similar gene expression patterns were observed at both 5% and 20% O2 for the remaining surface markers. Equine MSCs expressed the embryonic markers NANOG, OCT4 and SOX2 in both oxygen conditions. Additionally, hypoxic cells tended to display higher expression, which might indicate that hypoxia retains equine MSCs in an undifferentiated state. Conclusions Hypoxia attenuates the proliferative capacity of equine MSCs, but does not affect the phenotype and seems to keep them more undifferentiated than normoxic MSCs. 000148715 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000148715 590__ $$a1.861$$b2012 000148715 591__ $$aVETERINARY SCIENCES$$b18 / 142 = 0.127$$c2012$$dQ1$$eT1 000148715 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000148715 700__ $$0(orcid)0000-0002-1075-8267$$aRemacha, A. R. 000148715 700__ $$0(orcid)0000-0002-5748-6078$$aÁlvarez-Arguedas, S.$$uUniversidad de Zaragoza 000148715 700__ $$0(orcid)0000-0001-7188-0461$$aRomero, A.$$uUniversidad de Zaragoza 000148715 700__ $$0(orcid)0000-0002-8712-2275$$aVázquez, F. J.$$uUniversidad de Zaragoza 000148715 700__ $$0(orcid)0000-0001-5740-0185$$aZaragoza, P.$$uUniversidad de Zaragoza 000148715 700__ $$0(orcid)0000-0001-6016-4726$$aMartín-Burriel, I.$$uUniversidad de Zaragoza 000148715 700__ $$0(orcid)0000-0003-3289-2675$$aRodellar, C.$$uUniversidad de Zaragoza 000148715 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética 000148715 7102_ $$11001$$2X$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cProy. investigación DDA 000148715 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal 000148715 773__ $$g8 (2012), [12 pp.]$$pBMC Vet. Res.$$tBMC VETERINARY RESEARCH$$x1746-6148 000148715 8564_ $$s975007$$uhttps://zaguan.unizar.es/record/148715/files/texto_completo.pdf$$yVersión publicada 000148715 8564_ $$s2457045$$uhttps://zaguan.unizar.es/record/148715/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000148715 909CO $$ooai:zaguan.unizar.es:148715$$particulos$$pdriver 000148715 951__ $$a2025-01-21-14:44:01 000148715 980__ $$aARTICLE
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