148715 20250121150753.0 doi 10.1186/1746-6148-8-142 sideral 80193 ART-2012-80193 eng Ranera, B. Universidad de Zaragoza (orcid)0000-0002-4495-8857 Effect of hypoxia on equine mesenchymal stem cells derived from bone marrow and adipose tissue 2012 Background Mesenchymal stem cells (MSCs) derived from bone marrow (BM-MSCs) and adipose tissue (AT-MSCs) are being applied to equine cell therapy. The physiological environment in which MSCs reside is hypoxic and does not resemble the oxygen level typically used in in vitro culture (20% O2). This work compares the growth kinetics, viability, cell cycle, phenotype and expression of pluripotency markers in both equine BM-MSCs and AT-MSCs at 5% and 20% O2. Results At the conclusion of culture, fewer BM-MSCs were obtained in hypoxia than in normoxia as a result of significantly reduced cell division. Hypoxic AT-MSCs proliferated less than normoxic AT-MSCs because of a significantly higher presence of non-viable cells during culture. Flow cytometry analysis revealed that the immunophenotype of both MSCs was maintained in both oxygen conditions. Gene expression analysis using RT-qPCR showed that statistically significant differences were only found for CD49d in BM-MSCs and CD44 in AT-MSCs. Similar gene expression patterns were observed at both 5% and 20% O2 for the remaining surface markers. Equine MSCs expressed the embryonic markers NANOG, OCT4 and SOX2 in both oxygen conditions. Additionally, hypoxic cells tended to display higher expression, which might indicate that hypoxia retains equine MSCs in an undifferentiated state. Conclusions Hypoxia attenuates the proliferative capacity of equine MSCs, but does not affect the phenotype and seems to keep them more undifferentiated than normoxic MSCs. Access copy available to the general public Unrestricted info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 1.861 2012 VETERINARY SCIENCES 18 / 142 = 0.127 2012 Q1 T1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Remacha, A. R. (orcid)0000-0002-1075-8267 Álvarez-Arguedas, S. Universidad de Zaragoza (orcid)0000-0002-5748-6078 Romero, A. Universidad de Zaragoza (orcid)0000-0001-7188-0461 Vázquez, F. J. Universidad de Zaragoza (orcid)0000-0002-8712-2275 Zaragoza, P. Universidad de Zaragoza (orcid)0000-0001-5740-0185 Martín-Burriel, I. Universidad de Zaragoza (orcid)0000-0001-6016-4726 Rodellar, C. Universidad de Zaragoza (orcid)0000-0003-3289-2675 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 1001 X Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Proy. investigación DDA 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 8 (2012), [12 pp.] BMC Vet. Res. BMC VETERINARY RESEARCH 1746-6148 975007 http://zaguan.unizar.es/record/148715/files/texto_completo.pdf Versión publicada 2457045 http://zaguan.unizar.es/record/148715/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:148715 articulos driver 2025-01-21-14:44:01 ARTICLE