000148717 001__ 148717
000148717 005__ 20260112133146.0
000148717 0247_ $$2doi$$a10.1186/s40813-024-00380-y
000148717 0248_ $$2sideral$$a141772
000148717 037__ $$aART-2024-141772
000148717 041__ $$aeng
000148717 100__ $$aSagrera, Mònica
000148717 245__ $$aCan immunocrit be used as a monitoring tool for swine vaccination and infection studies?
000148717 260__ $$c2024
000148717 5060_ $$aAccess copy available to the general public$$fUnrestricted
000148717 5203_ $$aThe immunocrit is a cost-effective and straightforward technique traditionally used to assess passive immunity transfer to newborn piglets. However, it has not been previously used for monitoring the effect of vaccination and/or infections. Therefore, this study aimed to evaluate the usefulness of the immunocrit technique as an immunological monitoring tool in a vaccination and challenge scenario, using porcine circovirus 2 (PCV-2) as pathogen model. The immunocrit ratio was monitored in PCV-2 vaccinated (V) and non-vaccinated (NV) 3-week-old piglets (study day 0, SD0) that were subsequently challenged with this virus at SD21 and followed up to SD42. Additional techniques (PCV-2 IgG ELISA, optical refractometry, and proteinogram) were performed to further characterize the results of the immunocrit analysis.
Results
Immunocrit, γ-globulin concentration and PCV-2 S/P values followed similar dynamics: descending after PCV-2 vaccination but ascending after an experimental PCV-2 inoculation. However, statistically significant differences between V and NV animals were only found with the PCV-2 ELISA. In this case, V animals had significantly higher (p < 0.05) S/P values (S/P ratio = 0.74) than NV (S/P ratio = 0.39) pigs only after challenge at SD42. On the other hand, serum total protein obtained by refractometer (STPr) were maintained from SD0 to SD21 and increased in both groups from SD21 to SD42. Correlations between techniques were low to moderate, being the most robust ones found between immunocrit and optical refractometry (ρ = 0.41) and immunocrit with γ-globulins (ρ = 0.39). In a subset of sera, the proteinogram technique was applied to the whole serum and the supernatant of the immunocrit, with the objective to characterize indirectly the immunocrit fraction. The latter one included all protein types detectable through the proteinogram, with percentages varying between 64.3% (γ-globulins) and 82% (β-globulins).
Conclusion
The immunocrit technique represented a fraction of the total serum proteins, with low to moderate correlation with all the complementary techniques measured in this study. Its determination at different time points did not allow monitoring the effect of vaccination and/or infection using PCV-2 as a pathogen model.
000148717 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000148717 590__ $$a3.1$$b2024
000148717 592__ $$a0.776$$b2024
000148717 591__ $$aVETERINARY SCIENCES$$b14 / 170 = 0.082$$c2024$$dQ1$$eT1
000148717 593__ $$aAnimal Science and Zoology$$c2024$$dQ1
000148717 593__ $$aSmall Animals$$c2024$$dQ1
000148717 593__ $$aFood Animals$$c2024$$dQ1
000148717 594__ $$a5.7$$b2024
000148717 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000148717 700__ $$aSibila, Marina
000148717 700__ $$aMartínez-Boixaderas, Núria
000148717 700__ $$aLlorens, Anna Maria
000148717 700__ $$aEspigares, David
000148717 700__ $$aPastor, Josep
000148717 700__ $$0(orcid)0000-0001-7106-0068$$aGarza-Moreno, Laura$$uUniversidad de Zaragoza
000148717 700__ $$aSegalés, Joaquim
000148717 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000148717 773__ $$g10, 30 (2024), 9 pp.$$tPorcine Health Management$$x2055-5660
000148717 8564_ $$s1441201$$uhttps://zaguan.unizar.es/record/148717/files/texto_completo.pdf$$yVersión publicada
000148717 8564_ $$s2240673$$uhttps://zaguan.unizar.es/record/148717/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000148717 909CO $$ooai:zaguan.unizar.es:148717$$particulos$$pdriver
000148717 951__ $$a2026-01-12-12:36:35
000148717 980__ $$aARTICLE