148724 20250121150753.0 doi 10.1016/j.indcrop.2021.114413 sideral 128227 ART-2022-128227 eng Sanchez-Gomez, Susana Lethal and sublethal effects of essential oil-loaded zein nanocapsules on a zoonotic disease vector mosquito, and their non-target impact 2022 Recently the agrochemical industry is developing eco-friendly approaches based on the fabrication of biopesticides using substances, including biomass-borne products, with limited impact on human health and environment. One of these is the use of vegetable proteins, such as the corn-derived zein, as a safe nanocarrier loaded with bioactive compounds. Zein-based nanoparticles have been successfully applied to enhance the antimicrobial, antioxidant, repellent and insecticidal properties of hydrophobic substances such as essential oils (EOs). Herein, we developed two EO-loaded zein nanocapsules (NC) for mosquito larvicidal purposes. As active ingredients, we selected two Apiaceae essential oils (EO) with well documented insecticidal activities, i.e. Pimpinella anisum and Trachyspermum ammi, containing (E)-anethole and thymol as main constituents, respectively. The EO-based zein NC were prepared by self-assembly without using organic solvents, characterized for particle size by dynamic light scattering (DLS) and for stability over time and throughout stress conditions. EOloaded zein NC showed high acute toxicity on Culex quinquefasciatus 3rd instar larvae. Sublethal toxicity following 1-5 h to the LC90 was noted in terms of larval and pupal mortality, emerged adult rate, and sex ratio. The NC ecotoxicological safety was demonstrated, showing limited non-target effects on aquatic (Daphnia magna) and terrestrial (Eisenia fetida) invertebrates, as also on vertebrate cells (human keratinocytes). Overall, the P. anisum and T. ammi EO-loaded zein NC may represent effective and eco-friendly formulations to be considered in mosquito control programs, pending field evaluation of their epidemiological impact. info:eu-repo/grantAgreement/ES/MINECO/PGC2018-093789-B-I00 info:eu-repo/semantics/closedAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 5.9 2022 AGRONOMY 7 / 88 = 0.08 2022 Q1 T1 AGRICULTURAL ENGINEERING 3 / 14 = 0.214 2022 Q1 T1 0.897 2022 Agronomy and Crop Science 2022 Q1 9.7 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pagan, Rafael Universidad de Zaragoza (orcid)0000-0002-0238-6328 Pavela, Roman Mazzara, Eugenia Spinozzi, Eleonora Marinelli, Oliviero Zeppa, Laura Morshedloo, Mohammad Reza Maggi, Filippo Canale, Angelo Benelli, Giovanni 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 176 (2022), 114413 - [9 pp] Ind. crops prod. INDUSTRIAL CROPS AND PRODUCTS 0926-6690 993888 http://zaguan.unizar.es/record/148724/files/texto_completo.pdf Versión publicada 2415280 http://zaguan.unizar.es/record/148724/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:148724 articulos driver 2025-01-21-14:44:12 ARTICLE