000148743 001__ 148743 000148743 005__ 20250121150753.0 000148743 0247_ $$2doi$$a10.1016/j.ijfoodmicro.2015.06.030 000148743 0248_ $$2sideral$$a91483 000148743 037__ $$aART-2015-91483 000148743 041__ $$aeng 000148743 100__ $$aGomes Neto, N. J. 000148743 245__ $$aInfluence of general stress-response alternative sigma factors sS (RpoS) and sB (SigB) on bacterial tolerance to the essential oils from Origanum vulgare L. and Rosmarinus officinalis L. and pulsed electric fields 000148743 260__ $$c2015 000148743 5203_ $$aThis study assessed the influence of general stress-response alternative sigma factors RpoS (σS) and SigB (σB) on tolerance of Escherichia coli (E. coli MG1655 and its isogenic mutant E. coli MG1655 ΔrpoS) and Listeria monocytogenes (L. monocytogenes EGD-e and its isogenic mutant L. monocytogenes EGD-e ΔsigB) to the essential oils (EOs) from Origanum vulgare L.—oregano (OVEO) and Rosmarinus officinalis L.—rosemary (ROEO), as well as the changes in tolerance of parental and ΔrpoS and ΔsigB mutant strains to OVEO, ROEO and pulsed electric fields (PEF) following overnight exposure to subinhibitory concentrations (1/2 × minimum inhibitory concentration—MIC) of each tested EO. MIC values of OVEO and ROEO against the mutant cells were usually lower than those found against the parental cells. Survivor curves showed that mutant cells were more sensitive to these EOs than parental cells. The recovery of survivors in selective media showed a greater proportion of cells sublethally injured at their cell envelopes in the mutant strains compared with the parental strains. Induction of increased direct-tolerance to OVEO and ROEO or cross-tolerance to PEF was not observed after pre-exposure of parental and mutant cells to EOs. Otherwise, parental and mutant cells of E. coli and L. monocytogenes pre-exposed to OVEO or ROEO showed decreased tolerance when further treated with the homologous stressing agent at 2 × MIC. Still, mutant cells pre-exposed to OVEO or ROEO showed lower tolerance to PEF than parental strains. These results showed the influence of σS and σB in tolerance of single strains of E. coli and L. monocytogenes, respectively, to OVEO and ROEO. Moreover, the deletion of σS and σB resulted in decreased tolerance to OVEO, ROEO or PEF in tested strains following exposure to OVEO or ROEO at a subinhibitory concentration. 000148743 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/AGL2012-32165 000148743 540__ $$9info:eu-repo/semantics/closedAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/ 000148743 590__ $$a3.445$$b2015 000148743 591__ $$aFOOD SCIENCE & TECHNOLOGY$$b13 / 125 = 0.104$$c2015$$dQ1$$eT1 000148743 591__ $$aMICROBIOLOGY$$b39 / 123 = 0.317$$c2015$$dQ2$$eT1 000148743 592__ $$a1.614$$b2015 000148743 593__ $$aFood Science$$c2015$$dQ1 000148743 593__ $$aSafety, Risk, Reliability and Quality$$c2015$$dQ1 000148743 593__ $$aMicrobiology$$c2015$$dQ1 000148743 593__ $$aMedicine (miscellaneous)$$c2015$$dQ1 000148743 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000148743 700__ $$aMagnani, M. 000148743 700__ $$aChueca, B.$$uUniversidad de Zaragoza 000148743 700__ $$0(orcid)0000-0002-7629-8101$$aGarcía-Gonzalo, D.$$uUniversidad de Zaragoza 000148743 700__ $$0(orcid)0000-0002-0238-6328$$aPagán, R.$$uUniversidad de Zaragoza 000148743 700__ $$ade Souza, E. L. 000148743 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos 000148743 773__ $$g211 (2015), 32-37$$pInt. j. food microbiol.$$tINTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY$$x0168-1605 000148743 8564_ $$s475746$$uhttps://zaguan.unizar.es/record/148743/files/texto_completo.pdf$$yVersión publicada 000148743 8564_ $$s2902047$$uhttps://zaguan.unizar.es/record/148743/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000148743 909CO $$ooai:zaguan.unizar.es:148743$$particulos$$pdriver 000148743 951__ $$a2025-01-21-14:44:37 000148743 980__ $$aARTICLE