148785 20250121150754.0 doi 10.1016/j.ifset.2014.09.003 sideral 90328 ART-2015-90328 eng Chueca, B. Universidad de Zaragoza Transcriptomic analysis of Escherichia coli MG1655 cells exposed to pulsed electric fields 2015 DNA microarrays were used for the first time to study the mechanism of bacterial inactivation by pulsed electric fields (PEF). Escherichia coli MG1655 cells were PEF-treated for 50 pulses (0.08 Hz) at 20 kV/cm (2.10 kJ/kg per pulse) in buffer at pH 4.0 (100 μs PEF treatment time). After this PEF treatment, 47 genes demonstrated transcriptional differences. Among the 20 up-regulated genes, we found cytochrome bo oxidase genes (cyoB, cyoC, cyoD) and heme O synthase (cyoE), succinate dehydrogenase (sdhCDAB) operon, and transcriptional repressors of bet genes and chromosomal ars operon (betI and arsR, respectively). Gene class testing showed relevance of the tricarboxylic acid (TCA) cycle pathway, confirming cell requirement for energy and reducing power after PEF treatments. Transcriptomics after this PEF treatment were compared with cell transcriptome after a heat treatment using the same experimental setup, revealing a weaker cell response to PEF, which mainly involves components and functions directly associated with the cytoplasmic membrane. Resistance experiments confirmed results observed by transcriptomics, showing the lack of cross-resistance phenomena by PEF treatments. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/ES/MINECO/AGL2012-32165 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 2.997 2015 FOOD SCIENCE & TECHNOLOGY 18 / 125 = 0.144 2015 Q1 T1 1.675 2015 Chemistry (miscellaneous) 2015 Q1 Industrial and Manufacturing Engineering 2015 Q1 Food Science 2015 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Pagán, R. Universidad de Zaragoza (orcid)0000-0002-0238-6328 García-Gonzalo, D. Universidad de Zaragoza (orcid)0000-0002-7629-8101 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 29 (2015), 78-86 Innov. food sci. emerg. technol. INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES 1466-8564 1522265 http://zaguan.unizar.es/record/148785/files/texto_completo.pdf Postprint 602349 http://zaguan.unizar.es/record/148785/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:148785 articulos driver 2025-01-21-14:45:30 ARTICLE