148820 20250122151649.0 doi 10.3389/fmicb.2016.01845 sideral 108242 ART-2016-108242 eng Gayán, Elisa (orcid)0000-0002-5895-2157 Severely Heat Injured Survivors of E. coli O157:H7 ATCC 43888 Display Variable and Heterogeneous Stress Resistance Behavior 2016 Although minimal food processing strategies aim to eliminate foodborne pathogens and spoilage microorganisms through a combination of mild preservation techniques, little is actually known on the resistance behavior of the small fraction of microorganisms surviving an inimical treatment. In this study, the conduct of severely heat stressed survivors of E. coli O157:H7 ATCC 43888, as an indicator for the low infectious dose foodborne enterohemorrhagic strains, was examined throughout their resuscitation and outgrowth. Despite the fact that these survivors were initially sublethally injured, they were only marginally more sensitive to a subsequent heat treatment and actually much more resistant to a subsequent high hydrostatic pressure (HHP) shock in comparison with unstressed control cells. Throughout further resuscitation, however, their initial HHP resistance rapidly faded out, while their heat resistance increased and surpassed the initial heat resistance of unstressed control cells. Results also indicated that the population eventually emerging from the severely heat stressed survivors heterogeneously consisted of both growing and non-growing cells. Together, these observations provide deeper insights into the particular behavior and heterogeneity of stressed foodborne pathogens in the context of food preservation. Access copy available to the general public Unrestricted info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 4.076 2016 MICROBIOLOGY 25 / 122 = 0.205 2016 Q1 T1 1.758 2016 Microbiology (medical) 2016 Q1 Microbiology 2016 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Govers, Sander K. Michiels, Chris W. Aertsen, Abram 7 (2016), 1845 [8 pp] Front. microbiol. Frontiers in Microbiology 1664-302X 928865 http://zaguan.unizar.es/record/148820/files/texto_completo.pdf Versión publicada 2326880 http://zaguan.unizar.es/record/148820/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:148820 articulos driver 2025-01-22-14:45:57 ARTICLE