148848 20260217205523.0 doi 10.3390/ijms26010276 sideral 142049 ART-2024-142049 eng Díez-Sainz, Ester Human miR-1 Stimulates Metabolic and Thermogenic-Related Genes in Adipocytes 2024 Access copy available to the general public Unrestricted MicroRNAs play a pivotal role in the regulation of adipose tissue function and have emerged as promising therapeutic candidates for the management of obesity and associated comorbidities. Among them, miR-1 could be a potential biomarker for metabolic diseases and contribute to metabolic homeostasis. However, thorough research is required to fully elucidate the impact of miR-1 on human adipocyte thermogenesis and metabolism. This study aimed to explore the effect of miR-1 on human adipocyte browning, a process whose activation has been linked to obesity protection and counteraction. Human multipotent adipose-derived stem cells, hMADS cells, were differentiated into white and brown-like adipocytes and transfected with miR-1 mimics for gene expression and western blotting analyses. miR-1 inhibited the expression of its previously validated target PTK9/TWF1 and modulated the expression profile of key genes involved in thermogenesis and adipocyte browning (increased UCP1 at mRNA and protein level, increased CPT1M, decreased HIF3A), adipocyte differentiation and metabolism (decreased PLIN1, FASN, RXRA, PPARG, FABP4, MAPKAPK2), as well as genes related to the cytoskeleton (decreased ACTB) and extracellular matrix (decreased COL1A1). These findings suggest that miR-1 can modulate the expression of adipocyte human genes associated with thermogenesis and metabolism, which could hold value for eventual therapeutic potential in obesity. info:eu-repo/grantAgreement/ES/MICINN /BFU2015-65937-R info:eu-repo/grantAgreement/ES/MINECO/PID2022-141313OB-I00 info:eu-repo/grantAgreement/ES/MINECO/PID2022-141766OB-I00 info:eu-repo/grantAgreement/ES/MINECO/RTI2018-102205-B-I00 info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es 4.9 2024 BIOCHEMISTRY & MOLECULAR BIOLOGY 72 / 320 = 0.225 2024 Q1 T1 CHEMISTRY, MULTIDISCIPLINARY 70 / 239 = 0.293 2024 Q2 T1 1.273 2024 Medicine (miscellaneous) 2024 Q1 Physical and Theoretical Chemistry 2024 Q1 Computer Science Applications 2024 Q1 Inorganic Chemistry 2024 Q1 Spectroscopy 2024 Q1 Organic Chemistry 2024 Q1 Molecular Biology 2024 Q2 Catalysis 2024 Q2 9.0 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Milagro, Fermín I. Aranaz, Paula Riezu-Boj, José I. Batrow, Pierre-Louis Contu, Laura Gautier, Nadine Amri, Ez-Zoubir Mothe-Satney, Isabelle Lorente-Cebrián, Silvia Universidad de Zaragoza (orcid)0000-0002-1033-6152 1012 410 Universidad de Zaragoza Dpto. Farmac.Fisiol.y Med.L.F. Área Fisiología 26, 1 (2024), 276 [20 p.] Int. j. mol. sci. International Journal of Molecular Sciences 1661-6596 2355099 http://zaguan.unizar.es/record/148848/files/texto_completo.pdf Versión publicada 2605055 http://zaguan.unizar.es/record/148848/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:148848 articulos driver 2026-02-17-20:29:20 ARTICLE