000148906 001__ 148906
000148906 005__ 20250123152145.0
000148906 0247_ $$2doi$$a10.2147/VMRR.S306355
000148906 0248_ $$2sideral$$a124787
000148906 037__ $$aART-2021-124787
000148906 041__ $$aeng
000148906 100__ $$0(orcid)0000-0002-7822-6646$$aLacasta, Delia$$uUniversidad de Zaragoza
000148906 245__ $$aEffect of a Topical Formulation on Infective Viral Load in Lambs Naturally Infected with Orf Virus
000148906 260__ $$c2021
000148906 5060_ $$aAccess copy available to the general public$$fUnrestricted
000148906 5203_ $$aIntroduction: Orf is a highly contagious eruptive viral disease of the skin and mucosa of sheep and goats. Although vaccination with live or attenuated orf virus is the preferred option for disease control, the vaccine is unavailable in many countries. Treatment of orf lesions involves standard hygiene and in numerous cases, management of presumptive secondary infections with antibiotics, increasing risks of antimicrobial resistance (AMR). The wound dressing formulation Tri-Solfen® containing two local anaesthetics (lignocaine and bupivacaine), adrenaline and an antiseptic (cetrimide) in a gel formulation was developed for pain relief in sheep undergoing surgical husbandry procedures in Australia. Recently, TS therapy was found to reduce suffering and enhance recovery in cattle and buffalo with oral and skin lesions due to foot-and-mouth disease (FMD) virus infection. It was noted that TS has a low pH and is potentially viricidal, potentially aiding disease control.
Methods: One-month-old lambs (n=14), naturally infected with orf, were recruited from a farm during a natural outbreak of the disease. The animals were selected at the early stages of the infection and randomly divided into two cohorts: Group A (n=11) treated with the topical wound gel formulation (TS); and Group B (n=3) an untreated control group. Swabs were obtained before treatment (T0) and on days one (T1), 3 (T2) and 5 (T3) post-treatment, then submitted to direct DNA extraction with real-time PCR quantification, plus incubation with primary tissue cultures from ovine skin fibroblasts (OSF) and T-immortalized goat embryonic fibroblasts (TIGEF).
Results: Although no significant differences were found in the clinical progression of the lesions and PCR quantification (p=0.722) between these small cohorts, there was a significant difference (p< 0.05) in reduction in infective viral load between the groups when assessed in OSF cell cultures between T0 and T3.
Conclusion: These preliminary findings suggest that treatment of early stage lesions with this TS may reduce the infective viral load present in orf lesions.
000148906 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A15-17R
000148906 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/
000148906 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000148906 700__ $$aReina, Ramses
000148906 700__ $$0(orcid)0000-0002-7655-2472$$aRuiz de Arcaute, Marta$$uUniversidad de Zaragoza
000148906 700__ $$0(orcid)0000-0003-0042-8800$$aFerrer, Luis Miguel$$uUniversidad de Zaragoza
000148906 700__ $$0(orcid)0000-0002-2497-9028$$aBenito, Alfredo Angel
000148906 700__ $$0(orcid)0000-0001-5026-5144$$aTejedor, Maria Teresa$$uUniversidad de Zaragoza
000148906 700__ $$aEcheverria, Irache
000148906 700__ $$0(orcid)0000-0002-8474-2831$$aRuiz, Hector$$uUniversidad de Zaragoza
000148906 700__ $$aMartinez Cardenas, Silvia
000148906 700__ $$aWindsor, Peter Andrew
000148906 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000148906 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000148906 773__ $$g12 (2021), 149-158$$pVet. med.$$tVeterinary medicine$$x2230-2034
000148906 8564_ $$s617320$$uhttps://zaguan.unizar.es/record/148906/files/texto_completo.pdf$$yVersión publicada
000148906 8564_ $$s2512445$$uhttps://zaguan.unizar.es/record/148906/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000148906 909CO $$ooai:zaguan.unizar.es:148906$$particulos$$pdriver
000148906 951__ $$a2025-01-23-14:46:49
000148906 980__ $$aARTICLE