148910 20250123152145.0 doi 10.1016/j.meegid.2025.105714 sideral 142101 ART-2025-142101 eng Canuti, Marta Molecular ecology of novel amdoparvoviruses and old protoparvoviruses in Spanish wild carnivorans 2025 Wild carnivorans are key hosts of parvoviruses of relevance for animal health and wildlife conservation. However, the distribution and diversity of parvoviruses among wild carnivorans are under-investigated, particularly in Southern Europe. We evaluated the presence, spread, and diversity of multi-host protoparvoviruses (canine parvovirus type 2 (CPV-2), feline panleukopenia virus (FPV)), and amdoparvoviruses in 12 carnivoran species from Northern Spain to explore viral ecology. Broad-range PCRs were used to screen spleens (N = 157) and intestines (N = 116) from 171 road-killed mustelids, viverrids, and felids; identified viruses were molecularly characterized. We detected an Asian-like CPV-2c strain in the spleen of one wildcat (Felis silvestris, 1/40, 2.5 %), a globally distributed FPV strain in the spleen of one Eurasian badger (Meles meles, 1/35, 2.9 %), a novel amdoparvovirus (European mustelid amdoparvovirus 1), in the intestine and spleen of one stone marten (Martes foina, 1/16, 6.3 %) and in the spleen of one Eurasian badger (1/35, 2.9 %), the red fox fecal amdovirus (RFFAV) in the intestine and spleen of three wildcats (3/40, 7.5 %), and a novel amdoparvovirus closely related to RFFAV (European felid amdoparvovirus 1) in one wildcat (1/40, 2.5 %). We observed a correlation between the phylogeny of carnivorans and the one of amdoparvoviruses, possibly indicating virus-host co-evolution. Species originating from North America and Eurasia formed different clades, indicating local segregation in the absence of man-linked transboundary movements. In contrast, CPV-2 and FPV strains were internationally dispersed. Different parvovirus species co-occur in sympatric host populations, and higher viral diversity and additional hosts will likely be identified in future studies. Access copy available to the general public Unrestricted info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Mira, Francesco Villanúa, Diego Rodríguez-Pastor, Ruth (orcid)0000-0001-8435-6386 Guercio, Annalisa Urra, Fermín Millán, Javier (orcid)0000-0001-5608-781X 128 (2025), 105714 [11 pp.] INFECTION GENETICS AND EVOLUTION INFECTION GENETICS AND EVOLUTION 1567-1348 7068576 http://zaguan.unizar.es/record/148910/files/texto_completo.pdf Versión publicada 2398830 http://zaguan.unizar.es/record/148910/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:148910 articulos driver 2025-01-23-14:46:54 ARTICLE