000148918 001__ 148918
000148918 005__ 20250123152145.0
000148918 0247_ $$2doi$$a10.3389/fvets.2021.650572
000148918 0248_ $$2sideral$$a126599
000148918 037__ $$aART-2021-126599
000148918 041__ $$aeng
000148918 100__ $$aMendoza N.
000148918 245__ $$aInfluence of Non-conventional Sperm Quality Parameters on Field Fertility in Ovine
000148918 260__ $$c2021
000148918 5060_ $$aAccess copy available to the general public$$fUnrestricted
000148918 5203_ $$aThe prediction of the fertilizing ability of a seminal dose continues to be a primary aim in the field of artificial insemination (AI). To achieve this goal, in this study we have included the evaluation of some non-conventional sperm quality markers. A total of 3, 906 ewes from 52 different farms were inseminated with 357 refrigerated seminal doses obtained from 45 mature Rasa Aragonesa rams. The same samples were used for sperm quality analysis including membrane integrity, capacitation status, oxygen consumption and apoptotic-like markers such as phosphatidylserine translocation (PS), plasmalemma disorganization/mitochondrial membrane potential, caspase activation and DNA damage. Seminal doses from the breeding (B) season presented higher percentages of intact membrane (IM), non permeant (NP) membrane with high mitochondrial membrane potential (¿¿m) and IM without PS translocation spermatozoa than those from the non-breeding (NB) season. Therefore, we can conclude that there were less spermatozoa showing apoptotic-like features in the seminal doses from the B than the NB season, although these differences did not affect field fertility. Only the percentage of intact membrane, non-capacitated (IM-NC) spermatozoa showed a significant correlation with in vivo fertility (P = 0.005) and fecundity (P = 0.007) values obtained after cervical AI when all data were evaluated. When the data were sorted by season and distance to the farms where AI was performed, the correlation between the percentage of IM-NC spermatozoa and reproductive parameters increased in the NB season and progressively with remoteness from the farms. Some other sperm parameters, like NP with high ¿¿m, IM sperm without active caspases and DNA-intact spermatozoa, also showed significant correlations with the reproductive parameters in the sorted data. Moreover, the increment in both the percentage of IM-NC and DNA-intact spermatozoa would increase the probability of obtaining a fertility higher than the mean (>52%), as revealed by a multiple logistic regression analysis. In conclusion, we have identified two seminal markers—the percentage of intact membrane, non-capacitated spermatozoa, and DNA intact spermatozoa—which could be used as a test to discard males in AI programs, which is highly important from an economic point of view and can contribute to achieving satisfactory fertility rates. © Copyright © 2021 Mendoza, Casao, Domingo, Quintín, Laviña, Fantova, Cebrián-Pérez, Muiño-Blanco and Pérez-Pe.
000148918 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A26
000148918 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000148918 590__ $$a3.471$$b2021
000148918 591__ $$aVETERINARY SCIENCES$$b13 / 145 = 0.09$$c2021$$dQ1$$eT1
000148918 592__ $$a0.719$$b2021
000148918 593__ $$aVeterinary (miscellaneous)$$c2021$$dQ1
000148918 594__ $$a3.3$$b2021
000148918 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000148918 700__ $$0(orcid)0000-0003-1997-4262$$aCasao A.$$uUniversidad de Zaragoza
000148918 700__ $$aDomingo J.
000148918 700__ $$aQuintín F.
000148918 700__ $$aLaviña A.
000148918 700__ $$aFantova E.
000148918 700__ $$0(orcid)0000-0001-9842-6197$$aCebrián-Pérez J.Á.$$uUniversidad de Zaragoza
000148918 700__ $$0(orcid)0000-0003-1306-8517$$aMuiño-Blanco T.$$uUniversidad de Zaragoza
000148918 700__ $$0(orcid)0000-0002-2312-6402$$aPérez-Pe R.$$uUniversidad de Zaragoza
000148918 7102_ $$11002$$2819$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Zoología
000148918 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000148918 773__ $$g8 (2021), [10 pp]$$pFront. vet. sci.$$tFrontiers in Veterinary Science$$x2297-1769
000148918 8564_ $$s450113$$uhttps://zaguan.unizar.es/record/148918/files/texto_completo.pdf$$yVersión publicada
000148918 8564_ $$s2564531$$uhttps://zaguan.unizar.es/record/148918/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000148918 909CO $$ooai:zaguan.unizar.es:148918$$particulos$$pdriver
000148918 951__ $$a2025-01-23-14:47:04
000148918 980__ $$aARTICLE