000148962 001__ 148962
000148962 005__ 20250123152146.0
000148962 0247_ $$2doi$$a10.1016/j.impact.2024.100539
000148962 0248_ $$2sideral$$a142110
000148962 037__ $$aART-2025-142110
000148962 041__ $$aeng
000148962 100__ $$aBurgum, Michael J.
000148962 245__ $$aThe dispersion method does not affect the in vitro genotoxicity of multi-walled carbon nanotubes despite inducing surface alterations
000148962 260__ $$c2025
000148962 5060_ $$aAccess copy available to the general public$$fUnrestricted
000148962 5203_ $$aMulti-walled carbon nanotubes (MWCNTs) are a desirable class of high aspect ratio nanomaterials (HARNs) owing to their extensive applications. Given their demand, the growing occupational and consumer exposure to these materials has warranted an extensive investigation into potential hazards they may pose towards human health. This study utilised both the in vitro mammalian cell gene mutation and the cytokinesis-blocked micronucleus (CBMN) assays to investigate genotoxicity in human lymphoblastoid (TK6) and 16HBE14o− human lung epithelial cells, following exposure to NM-400 and NM-401 MWCNTs for 24 h. To evaluate the potential for secondary genotoxicity, the CBMN assay was applied on a co-culture of 16HBE14o− with differentiated human monocytic (dTHP-1) cells. In addition, two dispersion methods (NanoGenoTox vs. high shear mixing) were utilised prior to exposures and in acellular experiments to assess the effects on MWCNT oxidative potential, aspect ratio and surface properties. These were characterized in chemico as well as by electron microscopy and Raman spectroscopy. Structural damage of NM-400 was observed following both dispersion approaches; Raman spectra highlighted greater oxidative transformation under probe sonication as opposed to high shear mixing. Despite the changes to the oxidative potential of the MWCNTs, no statistically significant genotoxicity was observed under the conditions applied. There was also no visible signs of cellular internaliation of NM-400 or NM-401 into either cell type under the test conditions, which may support the negative genotoxic response. Whilst these HARNs may have oxidative potential, cells have natural protective mechanisms for repairing transient DNA damage. Therefore, it is crucial to evaluate biological endpoints which measure fixed DNA damage to account for the impact of DNA repair mechanisms.
000148962 536__ $$9info:eu-repo/grantAgreement/EC/H2020/814426/EU/Development and Implementation of a Sustainable Modelling Platform for NanoInformatics/NanoInformaTIX$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 814426-NanoInformaTIX
000148962 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000148962 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000148962 700__ $$aAlcolea-Rodríguez, Víctor
000148962 700__ $$aSaarelainen, Hanna
000148962 700__ $$aPortela, Raquel
000148962 700__ $$aReinosa, Julián J.
000148962 700__ $$aFernández, José F.
000148962 700__ $$aDumit, Verónica I.
000148962 700__ $$0(orcid)0000-0003-2936-242X$$aCatalán, Julia$$uUniversidad de Zaragoza
000148962 700__ $$aSimeone, Felice C.
000148962 700__ $$aFaccani, Lara
000148962 700__ $$aClift, Martin J.D.
000148962 700__ $$aEvans, Stephen J.
000148962 700__ $$aBañares, Miguel A.
000148962 700__ $$aDoak, Shareen H.
000148962 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000148962 773__ $$g37 (2025), 100539 [11 pp.]$$tNanoImpact$$x2452-0748
000148962 8564_ $$s3459535$$uhttps://zaguan.unizar.es/record/148962/files/texto_completo.pdf$$yVersión publicada
000148962 8564_ $$s2418734$$uhttps://zaguan.unizar.es/record/148962/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000148962 909CO $$ooai:zaguan.unizar.es:148962$$particulos$$pdriver
000148962 951__ $$a2025-01-23-14:47:56
000148962 980__ $$aARTICLE