000148968 001__ 148968
000148968 005__ 20250908131437.0
000148968 0247_ $$2doi$$a10.1016/j.jcpa.2024.11.002
000148968 0248_ $$2sideral$$a142120
000148968 037__ $$aART-2025-142120
000148968 041__ $$aeng
000148968 100__ $$0(orcid)0000-0002-2685-1730$$aRodríguez-Largo, Ana
000148968 245__ $$aMorphometry, cellular characterization and temporal evolution of granulomas induced by aluminium oxyhydroxide in sheep
000148968 260__ $$c2025
000148968 5060_ $$aAccess copy available to the general public$$fUnrestricted
000148968 5203_ $$aPersistent subcutaneous granulomas form at the injection site following administration of aluminium oxyhydroxide (AlOOH), a widely used vaccine adjuvant. Small ruminant lentiviruses (SRLVs) can infect macrophages within granulomas induced by commercial AlOOH-based vaccines in sheep. The entry of SRLVs into target cells involves the mannose receptor (MR), while catalytic polypeptide-like 3 protein containing Z1 domain (A3Z1) is considered a restriction factor for lentiviral replication. The objective of this study was to investigate the temporal evolution of AlOOH-induced post-vaccination granulomas in sheep experimentally infected with SRLVs. Twenty-four male lambs underwent two identical vaccination protocols and were challenged with SRLVs. Granulomas were detected in vaccinated groups only and progressively decreased in size. At post-mortem examination, 91.3% of the granulomas were recovered. Fistulas were present in granulomas following the second vaccination protocol. Central necrosis was present in 58.0% of granulomas and was associated with the vaccine used. Orthokeratotic keratin was seen within granulomas in 47.1% of the lambs. Considering all granulomas studied, significantly higher expression of MR was found compared with A3Z1. Differences in MR expression were related to the type of vaccine and the time since vaccination. A3Z1 expression was upregulated in granulomas from the infected groups. Macrophage polarization may influence SRLV infection of granulomas. While SRLV infection does not influence the architecture of post-vaccination granulomas, it may modulate their immune microenvironment. Further studies are needed to elucidate the complex interactions between AlOOH-induced granulomas and SRLV infection in sheep.
000148968 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A17-17R$$9info:eu-repo/grantAgreement/ES/MCIU/FPU18-01674$$9info:eu-repo/grantAgreement/ES/MCIU/RTI2018-096172-B-C33
000148968 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000148968 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000148968 700__ $$0(orcid)0000-0002-9723-9004$$aGómez, Álex$$uUniversidad de Zaragoza
000148968 700__ $$0(orcid)0009-0003-6592-6306$$aPérez, Estela$$uUniversidad de Zaragoza
000148968 700__ $$ade Miguel, Ricardo
000148968 700__ $$aMoncayola, Irati
000148968 700__ $$aBiagini, Lucia
000148968 700__ $$aRossi, Giacomo
000148968 700__ $$0(orcid)0000-0002-1204-4356$$ade Blas, Ignacio$$uUniversidad de Zaragoza
000148968 700__ $$0(orcid)0000-0002-2557-4890$$aFernández, Antonio$$uUniversidad de Zaragoza
000148968 700__ $$0(orcid)0000-0002-8133-2124$$aPérez, Marta$$uUniversidad de Zaragoza
000148968 700__ $$aGlaria, Idoia
000148968 700__ $$aReina, Ramsés
000148968 700__ $$0(orcid)0000-0002-2053-9842$$aLuján, Lluís$$uUniversidad de Zaragoza
000148968 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000148968 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000148968 7102_ $$11001$$2025$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Anatom.Anatom.Patológ.Com
000148968 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000148968 773__ $$g216 (2025), 1-9$$pJ. Comp. Pathol.$$tJOURNAL OF COMPARATIVE PATHOLOGY$$x0021-9975
000148968 8564_ $$s4677019$$uhttps://zaguan.unizar.es/record/148968/files/texto_completo.pdf$$yVersión publicada
000148968 8564_ $$s2639549$$uhttps://zaguan.unizar.es/record/148968/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000148968 909CO $$ooai:zaguan.unizar.es:148968$$particulos$$pdriver
000148968 951__ $$a2025-09-08-12:58:01
000148968 980__ $$aARTICLE