148968 20250908131437.0 doi 10.1016/j.jcpa.2024.11.002 sideral 142120 ART-2025-142120 eng Rodríguez-Largo, Ana (orcid)0000-0002-2685-1730 Morphometry, cellular characterization and temporal evolution of granulomas induced by aluminium oxyhydroxide in sheep 2025 Access copy available to the general public Unrestricted Persistent subcutaneous granulomas form at the injection site following administration of aluminium oxyhydroxide (AlOOH), a widely used vaccine adjuvant. Small ruminant lentiviruses (SRLVs) can infect macrophages within granulomas induced by commercial AlOOH-based vaccines in sheep. The entry of SRLVs into target cells involves the mannose receptor (MR), while catalytic polypeptide-like 3 protein containing Z1 domain (A3Z1) is considered a restriction factor for lentiviral replication. The objective of this study was to investigate the temporal evolution of AlOOH-induced post-vaccination granulomas in sheep experimentally infected with SRLVs. Twenty-four male lambs underwent two identical vaccination protocols and were challenged with SRLVs. Granulomas were detected in vaccinated groups only and progressively decreased in size. At post-mortem examination, 91.3% of the granulomas were recovered. Fistulas were present in granulomas following the second vaccination protocol. Central necrosis was present in 58.0% of granulomas and was associated with the vaccine used. Orthokeratotic keratin was seen within granulomas in 47.1% of the lambs. Considering all granulomas studied, significantly higher expression of MR was found compared with A3Z1. Differences in MR expression were related to the type of vaccine and the time since vaccination. A3Z1 expression was upregulated in granulomas from the infected groups. Macrophage polarization may influence SRLV infection of granulomas. While SRLV infection does not influence the architecture of post-vaccination granulomas, it may modulate their immune microenvironment. Further studies are needed to elucidate the complex interactions between AlOOH-induced granulomas and SRLV infection in sheep. info:eu-repo/grantAgreement/ES/DGA/A17-17R info:eu-repo/grantAgreement/ES/MCIU/FPU18-01674 info:eu-repo/grantAgreement/ES/MCIU/RTI2018-096172-B-C33 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Gómez, Álex Universidad de Zaragoza (orcid)0000-0002-9723-9004 Pérez, Estela Universidad de Zaragoza (orcid)0009-0003-6592-6306 de Miguel, Ricardo Moncayola, Irati Biagini, Lucia Rossi, Giacomo de Blas, Ignacio Universidad de Zaragoza (orcid)0000-0002-1204-4356 Fernández, Antonio Universidad de Zaragoza (orcid)0000-0002-2557-4890 Pérez, Marta Universidad de Zaragoza (orcid)0000-0002-8133-2124 Glaria, Idoia Reina, Ramsés Luján, Lluís Universidad de Zaragoza (orcid)0000-0002-2053-9842 2009 750 Universidad de Zaragoza Dpto. Química Analítica Área Química Analítica 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1001 025 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Anatom.Anatom.Patológ.Com 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 216 (2025), 1-9 J. Comp. Pathol. JOURNAL OF COMPARATIVE PATHOLOGY 0021-9975 4677019 http://zaguan.unizar.es/record/148968/files/texto_completo.pdf Versión publicada 2639549 http://zaguan.unizar.es/record/148968/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:148968 articulos driver 2025-09-08-12:58:01 ARTICLE