000149096 001__ 149096 000149096 005__ 20251017144549.0 000149096 0247_ $$2doi$$a10.1016/j.jprot.2012.02.025 000149096 0248_ $$2sideral$$a99976 000149096 037__ $$aART-2012-99976 000149096 041__ $$aeng 000149096 100__ $$aRamírez-Torres, A.$$uUniversidad de Zaragoza 000149096 245__ $$aProteomics and gene expression analyses of mitochondria from squalene-treated apoE-deficient mice identify short-chain specific acyl-CoA dehydrogenase changes associated with fatty liver amelioration 000149096 260__ $$c2012 000149096 5060_ $$aAccess copy available to the general public$$fUnrestricted 000149096 5203_ $$aSqualene, a hydrocarbon involved in cholesterol biosynthesis, is an abundant component in virgin olive oil. Previous studies showed that its administration decreased atherosclerosis and steatosis in male apoE knock-out mice. To study the effect of squalene on mitochondrial proteins in fatty liver, 1. g/kg/day of this isoprenoid was administered to those mice. After 10. weeks, hepatic fat was assessed and protein extracts from mitochondria enriched fractions from control and squalene-treated animals were analyzed by 2D-DIGE. Spots exhibiting significant differences were identified by MS analysis. Squalene administration modified the expression of eighteen proteins involved in different metabolic processes, 12 associated with hepatic fat content. Methionine adenosyltransferase I alpha (Mat1a) and short-chain specific acyl-CoA dehydrogenase (Acads) showed significant increased and decreased transcripts, respectively, consistent with their protein changes. These mRNAs were also studied in wild-type mice receiving squalene, where Mat1a was found increased and Acads decreased. However, this mRNA was significantly increased in the absence of apolipoprotein E. These results suggest that squalene action may be executed through a complex regulation of mitochondrial protein expression, including changes in Mat1a and Acads levels. Indeed, Mat1a is a target of squalene administration while Acads reflects the anti-steatotic properties of squalene. 000149096 536__ $$9info:eu-repo/grantAgreement/ES/CICYT-FEDER/SAF 2010-14958$$9info:eu-repo/grantAgreement/ES/DGA/PI025-08 000149096 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es 000149096 590__ $$a4.088$$b2012 000149096 591__ $$aBIOCHEMICAL RESEARCH METHODS$$b15 / 74 = 0.203$$c2012$$dQ1$$eT1 000149096 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000149096 700__ $$aBarceló-Batllori, S. 000149096 700__ $$aFernández-Vizarra, E. 000149096 700__ $$0(orcid)0000-0002-0108-1004$$aNavarro, M. A.$$uUniversidad de Zaragoza 000149096 700__ $$0(orcid)0000-0003-1197-4276$$aArnal, C.$$uUniversidad de Zaragoza 000149096 700__ $$0(orcid)0000-0001-6627-298X$$aGuillén, N.$$uUniversidad de Zaragoza 000149096 700__ $$aAcín, S. 000149096 700__ $$0(orcid)0000-0002-8251-8457$$aOsada, J.$$uUniversidad de Zaragoza 000149096 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal 000149096 7102_ $$11002$$2X$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cProy. investigación DEA 000149096 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole. 000149096 7102_ $$11000$$2807$$aUniversidad de Zaragoza$$bDpto. Anat.Pat.Med.Leg.For.To.$$cArea Toxicología 000149096 773__ $$g75, 9 (2012), 2563-2575$$pJ. proteomics$$tJOURNAL OF PROTEOMICS$$x1874-3919 000149096 8564_ $$s504161$$uhttps://zaguan.unizar.es/record/149096/files/texto_completo.pdf$$yVersión publicada 000149096 8564_ $$s1124354$$uhttps://zaguan.unizar.es/record/149096/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000149096 909CO $$ooai:zaguan.unizar.es:149096$$particulos$$pdriver 000149096 951__ $$a2025-10-17-14:11:03 000149096 980__ $$aARTICLE