000149143 001__ 149143
000149143 005__ 20251017144635.0
000149143 0247_ $$2doi$$a10.3897/zookeys.832.32257
000149143 0248_ $$2sideral$$a136047
000149143 037__ $$aART-2019-136047
000149143 041__ $$aeng
000149143 100__ $$aHernández Triana, Luis M.
000149143 245__ $$aDNA barcoding of British mosquitoes (Diptera, Culicidae) to support species identification, discovery of cryptic genetic diversity and monitoring invasive species.
000149143 260__ $$c2019
000149143 5060_ $$aAccess copy available to the general public$$fUnrestricted
000149143 5203_ $$aCorrect mosquito species identification is essential for mosquito and disease control programs. However, this is complicated by the difficulties in morphologically identifying some mosquito species. In this study, variation of a partial sequence of the cytochrome c oxidase unit I (COI) gene was used for the molecular identification of British mosquito species and to facilitate the discovery of cryptic diversity, and monitoring invasive species. Three DNA extraction methods were compared to obtain DNA barcodes from adult specimens. In total, we analyzed 42 species belonging to the genera Aedes Meigen, 1818 (21 species), Anopheles Meigen, 1818 (7 species), Coquillettidia Theobald, 1904 (1 species), Culex Linnaeus, 1758 (6 species), Culiseta Felt, 1904 (7 species), and Orthopodomyia Theobald, 1904 (1 species). Intraspecific genetic divergence ranged from 0% to 5.4%, while higher interspecific divergences were identified between Aedes geminus Peus, 1971/Culiseta litorea (Shute, 1928) (24.6%) and Ae. geminus/An. plumbeus Stephens, 1828 (22.5%). Taxonomic discrepancy was shown between An. daciae Linton, Nicolescu & Harbach, 2004 and An. messeae Falleroni, 1828 indicating the poor resolution of the COI DNA barcoding region in separating these taxa. Other species such as Ae. cantans (Meigen, 1818)/Ae. annulipes (Meigen, 1830) showed similar discrepancies indicating some limitation of this genetic marker to identify certain mosquito species. The combination of morphology and DNA barcoding is an effective approach for the identification of British mosquitoes, for invasive mosquitoes posing a threat to the UK, and for the detection of hidden diversity within species groups.
000149143 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000149143 590__ $$a1.137$$b2019
000149143 591__ $$aZOOLOGY$$b89 / 168 = 0.53$$c2019$$dQ3$$eT2
000149143 592__ $$a0.599$$b2019
000149143 593__ $$aEcology, Evolution, Behavior and Systematics$$c2019$$dQ2
000149143 593__ $$aAnimal Science and Zoology$$c2019$$dQ2
000149143 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000149143 700__ $$aBrugman, Victor A.
000149143 700__ $$aKikolova, Nadya I.
000149143 700__ $$0(orcid)0000-0001-8198-8118$$aRuiz Arrondo, Ignacio$$uUniversidad de Zaragoza
000149143 700__ $$aBarrero, Elsa
000149143 700__ $$aThorne, Leigh
000149143 700__ $$aFérnandez de Marco, Mar
000149143 700__ $$aKrüger, Andreas
000149143 700__ $$aLumley, Sarah
000149143 700__ $$aJohnson, Nicholas
000149143 700__ $$aFooks, Anthony R.
000149143 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000149143 773__ $$g832 (2019), 57-76$$pZOOKEYS$$tZooKeys$$x1313-2989
000149143 8564_ $$s1437659$$uhttps://zaguan.unizar.es/record/149143/files/texto_completo.pdf$$yVersión publicada
000149143 8564_ $$s1765519$$uhttps://zaguan.unizar.es/record/149143/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000149143 909CO $$ooai:zaguan.unizar.es:149143$$particulos$$pdriver
000149143 951__ $$a2025-10-17-14:29:01
000149143 980__ $$aARTICLE