000149834 001__ 149834
000149834 005__ 20250210083427.0
000149834 0247_ $$2doi$$a10.1016/j.actbio.2012.12.014
000149834 0248_ $$2sideral$$a81243
000149834 037__ $$aART-2013-81243
000149834 041__ $$aeng
000149834 100__ $$aAraña, M.
000149834 245__ $$aPreparation and characterization of collagen-based ADSC-carrier sheets for cardiovascular application
000149834 260__ $$c2013
000149834 5060_ $$aAccess copy available to the general public$$fUnrestricted
000149834 5203_ $$aThe use of scaffolds composed of natural biodegradable matrices represents an attractive strategy to circumvent the lack of cell engraftment, a major limitation of stem cell therapy in cardiovascular diseases. Bovine-derived non-porous collagen scaffolds with different degrees of cross-linking (C0, C2, C5 and C10) were produced and tested for their mechanical behavior, in vitro biocompatibility with adipose-derived stem cells (ADSCs) and tissue adhesion and inflammatory reaction. Uniaxial tensile tests revealed an anisotropic behavior of collagen scaffolds (2 × 0.5 cm) and statistically significant differences in the mechanical behavior between cross-linked and non-cross-linked scaffolds (n = 5). In vitro, ADSCs adhered homogenously and showed a similar degree of proliferation on all four types of scaffolds (cells × 103 cm−2 at day 7: C0: 94.7 ± 37.1; C2: 91.7 ± 25.6; C5: 88.2 ± 6.8; C10: 72.8 ± 10.7; P = n.s.; n = 3). In order to test the in vivo biocompatibility, a chronic myocardial infarction model was performed in rats and 1.2 × 1.2 cm size collagen scaffolds implanted onto the heart 1 month post-infarction. Six animals per group were killed 2, 7 and 30 days after transplant. Complete and long-lasting adhesion to the heart was only observed with the non-cross-linked scaffolds with almost total degradation 1 month post-transplantation. After 7 and 30 days post-implantation, the degree of inflammation was significantly lower in the hearts treated with non-cross-linked scaffolds (day 7: C0: 10.2 ± 2.1%; C2: 16.3 ± 2.9%; C5: 15.9 ± 4.8%; C10: 17.4 ± 4.1%; P < 0.05 vs. C0; day 30: C0: 1.3 ± 1.3%; C2: 9.4 ± 3.0%; C5: 7.0 ± 2.1%; C10: 9.8 ± 2.5%; P < 0.01 vs. C0). In view of the results, the non-cross-linked scaffold (C0) was chosen as an ADSC-carrier sheet and tested in vivo. One week post-implantation, 25.3 ± 7.0% of the cells transplanted were detected in those animals receiving the cell-carrier sheet whereas no cells were found in animals receiving cells alone (n = 3 animals/group).
We conclude that the biocompatibility and mechanical properties of the non-cross-linked collagen scaffolds make them a useful cell carrier that greatly favors tissue cell engraftment and may be exploited for cell transplantation in models of cardiac disease.
000149834 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000149834 590__ $$a5.684$$b2013
000149834 591__ $$aMATERIALS SCIENCE, BIOMATERIALS$$b3 / 29 = 0.103$$c2013$$dQ1$$eT1
000149834 591__ $$aENGINEERING, BIOMEDICAL$$b3 / 75 = 0.04$$c2013$$dQ1$$eT1
000149834 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000149834 700__ $$0(orcid)0000-0002-0664-5024$$aPeña, E.$$uUniversidad de Zaragoza
000149834 700__ $$aAbizanda, G.
000149834 700__ $$0(orcid)0000-0002-8503-9291$$aCilla, M.$$uUniversidad de Zaragoza
000149834 700__ $$0(orcid)0000-0003-2410-5678$$aOchoa, I.$$uUniversidad de Zaragoza
000149834 700__ $$aGavira, J. J.
000149834 700__ $$aEspinosa, G.
000149834 700__ $$0(orcid)0000-0001-8741-6452$$aDoblaré, M.$$uUniversidad de Zaragoza
000149834 700__ $$aPelacho, B.
000149834 700__ $$aProsper, F.
000149834 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología
000149834 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDpto. Ingeniería Mecánica$$cÁrea Mec.Med.Cont. y Teor.Est.
000149834 773__ $$g9, 4 (2013), 6075-6083$$pActa Biomater.$$tACTA BIOMATERIALIA$$x1742-7061
000149834 8564_ $$s6943525$$uhttps://zaguan.unizar.es/record/149834/files/texto_completo.pdf$$yPostprint
000149834 8564_ $$s2670534$$uhttps://zaguan.unizar.es/record/149834/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000149834 909CO $$ooai:zaguan.unizar.es:149834$$particulos$$pdriver
000149834 951__ $$a2025-02-10-08:31:08
000149834 980__ $$aARTICLE