149834 20251017144548.0 doi 10.1016/j.actbio.2012.12.014 sideral 81243 ART-2013-81243 eng Araña, M. Preparation and characterization of collagen-based ADSC-carrier sheets for cardiovascular application 2013 Access copy available to the general public Unrestricted The use of scaffolds composed of natural biodegradable matrices represents an attractive strategy to circumvent the lack of cell engraftment, a major limitation of stem cell therapy in cardiovascular diseases. Bovine-derived non-porous collagen scaffolds with different degrees of cross-linking (C0, C2, C5 and C10) were produced and tested for their mechanical behavior, in vitro biocompatibility with adipose-derived stem cells (ADSCs) and tissue adhesion and inflammatory reaction. Uniaxial tensile tests revealed an anisotropic behavior of collagen scaffolds (2 × 0.5 cm) and statistically significant differences in the mechanical behavior between cross-linked and non-cross-linked scaffolds (n = 5). In vitro, ADSCs adhered homogenously and showed a similar degree of proliferation on all four types of scaffolds (cells × 103 cm−2 at day 7: C0: 94.7 ± 37.1; C2: 91.7 ± 25.6; C5: 88.2 ± 6.8; C10: 72.8 ± 10.7; P = n.s.; n = 3). In order to test the in vivo biocompatibility, a chronic myocardial infarction model was performed in rats and 1.2 × 1.2 cm size collagen scaffolds implanted onto the heart 1 month post-infarction. Six animals per group were killed 2, 7 and 30 days after transplant. Complete and long-lasting adhesion to the heart was only observed with the non-cross-linked scaffolds with almost total degradation 1 month post-transplantation. After 7 and 30 days post-implantation, the degree of inflammation was significantly lower in the hearts treated with non-cross-linked scaffolds (day 7: C0: 10.2 ± 2.1%; C2: 16.3 ± 2.9%; C5: 15.9 ± 4.8%; C10: 17.4 ± 4.1%; P < 0.05 vs. C0; day 30: C0: 1.3 ± 1.3%; C2: 9.4 ± 3.0%; C5: 7.0 ± 2.1%; C10: 9.8 ± 2.5%; P < 0.01 vs. C0). In view of the results, the non-cross-linked scaffold (C0) was chosen as an ADSC-carrier sheet and tested in vivo. One week post-implantation, 25.3 ± 7.0% of the cells transplanted were detected in those animals receiving the cell-carrier sheet whereas no cells were found in animals receiving cells alone (n = 3 animals/group). We conclude that the biocompatibility and mechanical properties of the non-cross-linked collagen scaffolds make them a useful cell carrier that greatly favors tissue cell engraftment and may be exploited for cell transplantation in models of cardiac disease. info:eu-repo/semantics/openAccess by-nc-nd https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es 5.684 2013 MATERIALS SCIENCE, BIOMATERIALS 3 / 29 = 0.103 2013 Q1 T1 ENGINEERING, BIOMEDICAL 3 / 75 = 0.04 2013 Q1 T1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Peña, E. Universidad de Zaragoza (orcid)0000-0002-0664-5024 Abizanda, G. Cilla, M. Universidad de Zaragoza (orcid)0000-0002-8503-9291 Ochoa, I. Universidad de Zaragoza (orcid)0000-0003-2410-5678 Gavira, J. J. Espinosa, G. Doblaré, M. Universidad de Zaragoza (orcid)0000-0001-8741-6452 Pelacho, B. Prosper, F. 1003 443 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Histología 5004 605 Universidad de Zaragoza Dpto. Ingeniería Mecánica Área Mec.Med.Cont. y Teor.Est. 9, 4 (2013), 6075-6083 Acta Biomater. ACTA BIOMATERIALIA 1742-7061 6943525 http://zaguan.unizar.es/record/149834/files/texto_completo.pdf Postprint 2670534 http://zaguan.unizar.es/record/149834/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:149834 articulos driver 2025-10-17-14:10:39 ARTICLE