000149891 001__ 149891
000149891 005__ 20250128160611.0
000149891 0247_ $$2doi$$a10.1016/j.xpro.2023.102730
000149891 0248_ $$2sideral$$a137058
000149891 037__ $$aART-2023-137058
000149891 041__ $$aeng
000149891 100__ $$0(orcid)0000-0002-2954-1068$$aGarcía-Mendívil, Laura$$uUniversidad de Zaragoza
000149891 245__ $$aAutomatic quantification of myocardial remodeling features in human ventricular tissue from label-free microscopy
000149891 260__ $$c2023
000149891 5060_ $$aAccess copy available to the general public$$fUnrestricted
000149891 5203_ $$aThe procedures used routinely for collagen and lipofuscin evaluation are, in many cases, qualitative, observer dependent, and disregard spatial distribution. Here, we present a protocol for automatic quantification and spatial characterization of collagen and lipofuscin from label-free microscopy images of human ventricular tissues. We describe the steps for sample collection, tissue processing, image acquisition, and quantification of collagen and lipofuscin. This protocol avoids discrepancies between observers and can be adapted to other tissues and species.
For complete details on the use and execution of this protocol, please refer to García-Mendívil et al. (2022).
000149891 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000149891 592__ $$a0.955$$b2023
000149891 593__ $$aImmunology and Microbiology (miscellaneous)$$c2023$$dQ1
000149891 593__ $$aNeuroscience (miscellaneous)$$c2023$$dQ2
000149891 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2023$$dQ2
000149891 594__ $$a2.0$$b2023
000149891 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000149891 700__ $$0(orcid)0000-0002-3194-7796$$aPérez-Zabalza, María
000149891 700__ $$aDuwé, Sam
000149891 700__ $$0(orcid)0000-0003-3982-1263$$aOrdovás, Laura
000149891 700__ $$0(orcid)0000-0002-1960-407X$$aPueyo, Esther$$uUniversidad de Zaragoza
000149891 7102_ $$15008$$2800$$aUniversidad de Zaragoza$$bDpto. Ingeniería Electrón.Com.$$cÁrea Teoría Señal y Comunicac.
000149891 773__ $$g4, 4 (2023), 102730 [14 pp.]$$tSTAR Protocols$$x2666-1667
000149891 8564_ $$s4611391$$uhttps://zaguan.unizar.es/record/149891/files/texto_completo.pdf$$yVersión publicada
000149891 8564_ $$s1487216$$uhttps://zaguan.unizar.es/record/149891/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000149891 909CO $$ooai:zaguan.unizar.es:149891$$particulos$$pdriver
000149891 951__ $$a2025-01-28-14:58:07
000149891 980__ $$aARTICLE